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The Griess test is an analytical chemistry test which detects the presence of nitrite ion in solution. One of its most important uses is the determination of nitrite in drinking water . The Griess diazotization reaction, on which the Griess reagent relies, was first described in 1858 by Peter Griess .
Colorimetric analysis is a method of determining the concentration of a chemical element or chemical compound in a solution with the aid of a color reagent. It is applicable to both organic compounds and inorganic compounds and may be used with or without an enzymatic stage.
A nitrite test is a standard component of a urinary test strip. A positive test for nitrites in the urine is called nitrituria. This test is commonly used in diagnosing urinary tract infections (UTIs). A positive nitrite test indicates that the cause of the UTI is a Gram-negative organism, most commonly Escherichia coli. The reason for nitrites ...
Colorimetric assays use reagents that undergo a measurable color change in the presence of the analyte. They are widely used in biochemistry to test for the presence of enzymes, specific compounds, antibodies, hormones and many more analytes. For example, para-Nitrophenylphosphate is converted into a yellow product by alkaline phosphatase enzyme.
N-(1-Naphthyl)ethylenediamine dihydrochloride is widely used in the quantitative analysis of nitrate and nitrite in water samples by colorimetry. It readily undergoes a diazonium coupling reaction in the presence of nitrite to give a strongly colored azo compound .
An ion selective electrode, or distillation/titration method can often be used in cases where Berthelot chemistry is ineffective. Berthelot chemistry has also been adapted for the analysis of nitrite and nitrate in soil and water after conversion, typically by reduction with Devarda's alloy, of these species to ammonium. [3]
An EPA scientist samples water in Florida Everglades. Samples of water from the natural environment are routinely taken and analyzed as part of a pre-determined monitoring program by regulatory authorities to ensure that waters remain unpolluted, or if polluted, that the levels of pollution are not increasing or are falling in line with an agreed remediation plan.
Several conventional colorimetric analyses have been miniaturized to function quantitatively in a plate reader, with performance suitable for research purposes. Examples of analyses converted to plate reader methods include several for ammonium , nitrate , nitrite , [ 4 ] urea , [ 5 ] iron(II), [ 6 ] and orthophosphate . [ 7 ]
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