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p-Dimethylaminobenzophenone is related to Michler's ketone, but with only one amine. [5] Auramine O, a dye, is a salt of the iminium cation [(CH 3) 2 NC 6 H 4] 2 CNH 2 +.Michler's thione, [(CH 3) 2 NC 6 H 4] 2 CS, is prepared by treatment of Michler's ketone with hydrogen sulfide in the presence of acid or sulfideing auramine O. [6] Hydride reduction of Michler's ketone gives 4,4'-bis ...
It is colored yellow below pH 5.2, and violet above pH 6.8. In its cyclic sulfonate ester form, it has a p K a value of 6.3, and is usually prepared as a 0.04% aqueous solution. [ 1 ]
After experiments, researchers found that a solution containing: 85.9 % water; 10 % glycerol; 4 % dishwashing liquid; ... Crystal violet lactone is an example.
Azo violet (Magneson I; [1] p-nitrobenzeneazoresorcinol) is an azo compound with the chemical formula C 12 H 9 N 3 O 4. It is used commercially as a violet dye and experimentally as a pH indicator, appearing yellow below pH 11, and violet above pH 13. [2] It also turns deep blue in the presence of magnesium salt in a slightly alkaline, or basic ...
Bromothymol blue is synthesized by addition of elemental bromine to thymol blue in a solution in glacial acetic acid. [6] To prepare a solution for use as pH indicator, dissolve 0.10 g in 8.0 cm 3 N/50 (a.k.a. 0.02 Normal) NaOH and dilute with water to 250 cm 3.
Resazurin (7-Hydroxy-3H-phenoxazin-3-one 10-oxide) is a phenoxazine dye that is weakly fluorescent, nontoxic, cell-permeable, and redox‐sensitive. [2] [3] Resazurin has a blue to purple color above pH 6.5 and an orange color below pH 3.8. [4]
A solution of iodine I 2 is yellow/brown in color. When this is added to a solution to be tested, however, any chemical group (usually in this test −C=C− double bonds) that react with iodine effectively reduce the strength, or magnitude of the color (by taking I 2 out of solution). Thus the amount of iodine required to make a solution ...
There are many different ways to prepare PBS solutions, common ones are Dulbecco's phosphate-buffered saline (DPBS) [2] and the Cold Spring Harbor protocol. [3] Some formulations of DPBS do not contain potassium and magnesium, while other ones contain calcium and/or magnesium (depending on whether or not the buffer is used on live or fixed tissue: the latter does not require CaCl 2 or MgCl 2).