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A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue .
It describes the appearance of cells, tissues and cellular structures as seen through the microscope after a histological section has been stained with a basic dye. The most common such dye is haematoxylin. The name basophilic refers to the characteristic of these structures to be stained very well by basic dyes.
The main parts of the Jupyter Notebooks are: Metadata, Notebook format and list of cells. Metadata is a data Dictionary of definitions to set up and display the notebook. Notebook Format is a version number of the software. List of cells are different types of Cells for Markdown (display), Code (to execute), and output of the code type cells. [23]
A "vital dye" or stain is a dye capable of penetrating living cells or tissues without causing immediate visible degenerative changes. [26] Such dyes are useful in medical and pathological fields in order to selectively color certain structures (such as cells) in order to distinguish them from surrounding tissue and thus make them more visible ...
In contrast, basic dyes are used to stain cell nuclei and some other acidic components of tissues. [8] Regarding cellular structures, acid dyes will stain acidophilic structures that have a net positive charge due to the fact that they have a negatively charged chromophore. Acidophilic structures include the cytoplasm, collagen and mitochondria ...
Main staining types when using hematoxylin and eosin (H&E), where acidophile cells stain eosinophilic. Acidophile (or acidophil, or, as an adjectival form, acidophilic) is a term used by histologists to describe a particular staining pattern of cells and tissues when using haematoxylin and eosin stains. Specifically, the name refers to ...
Gram-positive cells have a thick layer of peptidoglycan in the cell wall that retains the primary stain, crystal violet. Gram-negative cells have a thinner peptidoglycan layer that allows the crystal violet to wash out on addition of ethanol. They are stained pink or red by the counterstain, [3] commonly safranin or fuchsine.
Immunocytochemistry is a technique used to assess the presence of a specific protein or antigen in cells (cultured cells, cell suspensions) by use of a specific antibody, which binds to it, thereby allowing visualization and examination under a microscope. It is a valuable tool for the determination of cellular contents from individual cells.