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27226 Ensembl ENSG00000146070 ENSMUSG00000023913 UniProt Q13093 Q60963 RefSeq (mRNA) NM_001168357 NM_005084 NM_013737 RefSeq (protein) NP_001161829 NP_005075 NP_038765 Location (UCSC) Chr 6: 46.7 – 46.74 Mb Chr 17: 43.88 – 43.92 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Lipoprotein-associated phospholipase A 2 (Lp-PLA 2) also known as platelet-activating factor ...
BCA protein assay in a 96 well plate. The bicinchoninic acid assay (BCA assay), also known as the Smith assay, after its inventor, Paul K. Smith at the Pierce Chemical Company, [1] now part of Thermo Fisher Scientific, is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL), similar to Lowry protein assay, Bradford protein assay or ...
This method is able to detect as low as 25 μg/ml and up to 2000 μg/ml of protein in a 65 ul sample, using standard protocol. This method may be preferred for samples containing detergents or other reducing agents. This method has a fast detection speed and low protein-to-protein variability in comparison to the BCA or Coomassie (Bradford ...
Since the first publication by Kansy and coworkers, [7] several companies developed their own versions of the assay. Early models incorporated iso-pH conditions in the compartments separated by a simple lipid membrane; subsequently, commercial products were introduced which incorporated more sophisticated lipid membranes. [8]
Protein–lipid interaction is the influence of membrane proteins on the lipid physical state or vice versa. The questions which are relevant to understanding of the structure and function of the membrane are: 1) Do intrinsic membrane proteins bind tightly to lipids (see annular lipid shell ), and what is the nature of the layer of lipids ...
Lipids having a conical molecular shape alter the energetic state of membrane cholesterol, augmenting the interaction of the sterol with the cholesterol-specific cytolysin. [30] Since high cholesterol concentrations are required for CDC binding/pore-formation, it was thought that CDC would associate with lipid rafts .
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