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The biological replicates include independent RNA extractions. Technical replicates may be two aliquots of the same extraction. Third, spots of each cDNA clone or oligonucleotide are present as replicates (at least duplicates) on the microarray slide, to provide a measure of technical precision in each hybridization.
In the biological sciences, replicates are an experimental units that are treated identically. Replicates are an essential component of experimental design because ...
Subsequent technological advances since the late 1990s have repeatedly transformed the field and made transcriptomics a widespread discipline in biological sciences. There are two key contemporary techniques in the field: microarrays , which quantify a set of predetermined sequences, and RNA-Seq , which uses high-throughput sequencing to record ...
BioJupies is a web-based platform that provides complete RNA-seq analysis solution from free alignment service to a complete data analysis report delivered as an interactive Jupyter Notebook. BioQueue is a web-based queue engine designed preferentially to improve the efficiency and robustness of job execution in bioinformatics research by ...
Sacrificial pseudoreplication (Figure 5b in Hurlbert 1984) occurs when means within a treatment are used in an analysis, and these means are tested over the within unit variance. In Figure 5b the erroneous F-ratio will have 1 df in the numerator (treatment) mean square and 4 df in the denominator mean square(2-1 = 1 df for each experimental unit).
In engineering, science, and statistics, replication is the process of repeating a study or experiment under the same or similar conditions to support the original claim, which is crucial to confirm the accuracy of results as well as for identifying and correcting the flaws in the original experiment. [1]
Data generation artifacts (also known as technical variance): The reagents (e.g., library preparation kit), personnel involved, and type of sequencer (e.g., Illumina, Pacific Biosciences) can result in technical artifacts that might be mis-interpreted as meaningful results. As with any scientific experiment, it is prudent to conduct RNA-Seq in ...
Biological network inference is the process of making inferences and predictions about biological networks. [1] By using these networks to analyze patterns in biological systems, such as food-webs, we can visualize the nature and strength of these interactions between species, DNA, proteins, and more.