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  2. Etest - Wikipedia

    en.wikipedia.org/wiki/Etest

    Etest is a quantitative technique for determining the MIC of microoganisms. It is used for a range of Gram-negative and Gram-positive bacteria such as Pseudomonas, [2] [3] Staphylococcus, [4] and Enterococcus species, [5] as well as fastidious bacteria, such as Neisseria and Streptococcus pneumoniae. [1]

  3. Gram stain - Wikipedia

    en.wikipedia.org/wiki/Gram_stain

    The small oval chlamydospores are 2–4 μm in diameter. Gram staining is a bacteriological laboratory technique [8] used to differentiate bacterial species into two large groups (gram-positive and gram-negative) based on the physical properties of their cell walls. [9] [page needed] Gram staining can also be used to diagnose a fungal infection ...

  4. McFarland standards - Wikipedia

    en.wikipedia.org/wiki/McFarland_standards

    A 0.5 McFarland standard is prepared by mixing 0.05 mL of 1.175% barium chloride dihydrate (BaCl 2 •2H 2 O), with 9.95 mL of 1% sulfuric acid (H 2 SO 4). [ 1 ] Now there are McFarland standards prepared from suspensions of latex particles, which lengthens the shelf life and stability of the suspensions.

  5. Diagnostic microbiology - Wikipedia

    en.wikipedia.org/wiki/Diagnostic_Microbiology

    Diagnostic microbiology is the study of microbial identification. Since the discovery of the germ theory of disease , scientists have been finding ways to harvest specific organisms. Using methods such as differential media or genome sequencing , physicians and scientists can observe novel functions in organisms for more effective and accurate ...

  6. ATP test - Wikipedia

    en.wikipedia.org/wiki/ATP_test

    ATP is a molecule found only in and around living cells, and as such it gives a direct measure of biological concentration and health. ATP is quantified by measuring the light produced through its reaction with the naturally-occurring firefly enzyme luciferase using a luminometer.

  7. Antibiotic sensitivity testing - Wikipedia

    en.wikipedia.org/wiki/Antibiotic_sensitivity_testing

    Using magnetic nanoparticles studded with a beta-2-glycoprotein I peptide imitating a plasma protein, microbial pathogens could selectively be retrieved from blood culture specimens within hours, in a study published September 2024. Magnets are used to fish out the peptide-bacterial complex, followed by genetic testing.

  8. IMViC - Wikipedia

    en.wikipedia.org/wiki/IMViC

    The solution remaining yellow (pH = 6.2 or above) indicates a negative test, meaning the butanediol fermentation is used. The VP test uses alpha-naphthol and potassium hydroxide to test for the presence of acetylmethylcarbinol (acetoin), an intermediate of the 2,3-butanediol fermentation pathway. After adding both reagents, the tube is shaken ...

  9. Bergey's Manual of Systematic Bacteriology - Wikipedia

    en.wikipedia.org/wiki/Bergey's_Manual_of...

    Bergey's Manual Trust was established in 1936 to sustain the publication of Bergey's Manual of Determinative Bacteriology and supplementary reference works. The Trust also recognizes individuals who have made outstanding contributions to bacterial taxonomy by presentation of the Bergey Award and Bergey Medal, jointly supported by funds from the Trust and from Springer, the publishers of the ...