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  2. Bacteriophage - Wikipedia

    en.wikipedia.org/wiki/Bacteriophage

    An example of a bacteriophage known to follow the lysogenic cycle and the lytic cycle is the phage lambda of E. coli. [53] Sometimes prophages may provide benefits to the host bacterium while they are dormant by adding new functions to the bacterial genome , in a phenomenon called lysogenic conversion .

  3. Filamentous bacteriophage - Wikipedia

    en.wikipedia.org/wiki/Filamentous_bacteriophage

    During fd phage assembly, the phage DNA is first packaged into a linear intracellular nucleoprotein complex with many copies of the phage gene 5 replication/assembly protein. The gene 5 protein is then displaced by the gene 8 coat protein as the nascent phage is extruded across the bacterial plasma membrane without killing the bacterial host.

  4. Phagemid - Wikipedia

    en.wikipedia.org/wiki/Phagemid

    The 'helper' phage infects the bacterial host by first attaching to the host cell's pilus and then, after attachment, transporting the phage genome into the cytoplasm of the host cell. Inside the cell, the phage genome triggers production of single stranded phagemid DNA in the cytoplasm. This phagemid DNA is then packaged into phage particles.

  5. Lysogenic cycle - Wikipedia

    en.wikipedia.org/wiki/Lysogenic_cycle

    3. The phage DNA then moves through the cell to the host's DNA. 4. The phage DNA integrates itself into the host cell's DNA, creating prophage. 5. The prophage then remains dormant until the host cell divides. 6. After the host cell has divided, the phage DNA in the daughter cells activate, and the phage DNA begins to express itself.

  6. Lambda phage - Wikipedia

    en.wikipedia.org/wiki/Lambda_phage

    Lambda phage is a non-contractile tailed phage, meaning during an infection event it cannot 'force' its DNA through a bacterial cell membrane. It must instead use an existing pathway to invade the host cell, having evolved the tip of its tail to interact with a specific pore to allow entry of its DNA to the hosts.

  7. Electroporation - Wikipedia

    en.wikipedia.org/wiki/Electroporation

    For example, it is used in the process of producing knockout mice, as well as in tumor treatment, gene therapy, and cell-based therapy. The process of introducing foreign DNA into eukaryotic cells is known as transfection. Electroporation is highly effective for transfecting cells in suspension using electroporation cuvettes.

  8. Transduction (genetics) - Wikipedia

    en.wikipedia.org/wiki/Transduction_(genetics)

    An example is the viral transfer of DNA from one bacterium to another and hence an example of horizontal gene transfer. [ 2 ] [ 3 ] [ page needed ] Transduction does not require physical contact between the cell donating the DNA and the cell receiving the DNA (which occurs in conjugation ), and it is DNase resistant ( transformation is ...

  9. P1 phage - Wikipedia

    en.wikipedia.org/wiki/P1_phage

    The phage particle adsorbs onto the surface of the bacterium using the tail fibers for specificity. The tail sheath contracts and the DNA of the phage is injected into the host cell. The host DNA recombination machinery or the cre enzyme translated from the viral DNA recombine the terminally redundant ends and circularize the genome.