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Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is considered a housekeeping gene. For this reason, GAPDH is commonly used by biological researchers as a loading control for western blot and as a control for qPCR.
Housekeeping genes account for majority of the active genes in the genome, and their expression is obviously vital to survival. The housekeeping gene expression levels are fine-tuned to meet the metabolic requirements in various tissues.
The following represent genes that should probably not be used for reference purposes: GUSB, [5] RPLP0, [5] TFRC, [5] GAPDH, HSP90, and β-actin. Although they were once considered as "housekeeping genes," recent data suggests that they are not as reliable as once thought. [1] [2]
Housekeeping genes and proteins, including β-Actin, GAPDH, HPRT1, and RPLP1, are often used as internal controls in western blots because they are thought to be expressed constitutively, at the same levels, across experiments.
The bird flu outbreak has taken concerning turns, with more than 60 human cases confirmed. Experts outlined four signs that the virus is going in the wrong direction.
The number of migrants arrested illegally crossing the U.S.-Mexico border in December was lower than when President-elect Donald Trump ended his first term in 2020, according to preliminary ...
These genes are often selected from housekeeping genes as their functions related to basic cellular survival normally imply constitutive gene expression. [ 5 ] [ 6 ] This enables researchers to report a ratio for the expression of the genes of interest divided by the expression of the selected normalizer, thereby allowing comparison of the ...
Two Indian chemical companies have been indicted for allegedly importing ingredients for the highly addictive opioid fentanyl into the United States and Mexico, the U.S. Department of Justice said ...
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