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A centrifuge is a device that uses centrifugal force to subject a specimen to a specified constant force - for example, to separate various components of a fluid. This is achieved by spinning the fluid at high speed within a container, thereby separating fluids of different densities (e.g. cream from milk) or liquids from solids. It works by ...
It is the perpendicular force exerted on the contents of the rotor as a result of the rotation, always relative to the gravity of the Earth, which measures the strength of rotors of different types and sizes. For instance, the RCF of 1000 x g means that the centrifugal force is 1000 times stronger than the Earth's gravitational force.
Centrifugal force is a fictitious force in Newtonian mechanics (also called an "inertial" or "pseudo" force) that appears to act on all objects when viewed in a rotating frame of reference. It appears to be directed radially away from the axis of rotation of the frame.
Diagram of a gas centrifuge with countercurrent flow, used for separating isotopes of uranium. A gas centrifuge is a device that performs isotope separation of gases. A centrifuge relies on the principles of centrifugal force accelerating molecules so that particles of different masses are physically separated in a gradient along the radius of a rotating container.
Gravitational force; Difference in density; Fluid viscosity; Particle size and shape; Larger particles sediment more quickly and at lower centrifugal forces. If a particle is less dense than the fluid (e.g., fats in water), the particle will not sediment, but rather will float, regardless of strength of the g-force experienced by the particle.
Rotor failure, caused by mechanical stress from the high forces imparted by the motor, can occur due to manufacturing defects, routine wear and tear, or improper use and maintenance. Such a failure can be catastrophic failure, especially with larger centrifuges, and generally results in total destruction of the centrifuge. While centrifuges ...
The force interacts with a property of the sample, thereby the separation occurs, in other words, the components show differing "mobilities" under the force exerted by the crossing field. As an example, for the hydraulic, or cross-flow FFF method, the property driving separation is the translational diffusion coefficient or the hydrodynamic size.
The centrifugal force is given by the equation: = where m is the excess mass of the particle over and above the mass of an equivalent volume of the fluid in which the particle is situated (see Archimedes' principle) and r is the distance of the particle from the axis of rotation. When the two opposing forces, viscous and centrifugal, balance ...