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Salmonella species can be found in the digestive tracts of humans and animals, especially reptiles. Salmonella on the skin of reptiles or amphibians can be passed to people who handle the animals. [39] Food and water can also be contaminated with the bacteria if they come in contact with the feces of infected people or animals. [40]
Immunological methods using monoclonal antibodies can be used to detect indicator bacteria in water samples. Precultivation in select medium must preface detection to avoid detection of dead cells. ELISA antibody technology has been developed to allow for readable detection by the naked eye for rapid identification of coliform microcolonies.
Detection of viral RNA and DNA genomes can be performed using polymerase chain reaction. This technique makes many copies of the virus genome using virus-specific probes. Variations of PCR such as nested reverse transcriptase PCR and real time PCR can also be used to determine viral loads in patient serum.
Bacteria, viruses or fungi can contaminate food and make people sick. "The three we see the most often (in the U.S.) are E coli, listeria and salmonella," says Detwiler.
Salmonellosis is a symptomatic infection caused by bacteria of the Salmonella type. [1] It is the most common disease to be known as food poisoning (though the name refers to food-borne illness in general), these are defined as diseases, usually either infectious or toxic in nature, caused by agents that enter the body through the ingestion of food.
Nucleic acid sequence-based amplification, commonly referred to as NASBA, is a method in molecular biology which is used to produce multiple copies of single stranded RNA. [1] NASBA is a two-step process that takes RNA and anneals specially designed primers, then utilizes an enzyme cocktail to amplify it.
Phage typing is a phenotypic method that uses bacteriophages ("phages" for short) for detecting and identifying single strains of bacteria. [1] Phages are viruses that infect bacteria and may lead to bacterial cell lysis. [2] The bacterial strain is assigned a type based on its lysis pattern. [3]
A Salmonella serotype is determined by the unique combination of reactions of cell surface antigens. For Salmonella, the O and H antigens are used. [22] There are two species of Salmonella: Salmonella bongori and Salmonella enterica. Salmonella enterica can be subdivided into six subspecies. The process to identify the serovar of the bacterium ...
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