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Binary Alignment Map (BAM) is the comprehensive raw data of genome sequencing; [1] it consists of the lossless, compressed binary representation of the Sequence Alignment Map-files. [2] [3] BAM is the compressed binary representation of SAM (Sequence Alignment Map), a compact and index-able representation of nucleotide sequence alignments. [4]
SAMtools makes it possible to work directly with a compressed BAM file, without having to uncompress the whole file. Additionally, since the format for a SAM/BAM file is somewhat complex - containing reads, references, alignments, quality information, and user-specified annotations - SAMtools reduces the effort needed to use SAM/BAM files by ...
Raw PacBio subreads use the same convention but typically assign a placeholder base quality (Q0) to all bases in the read. [7] Oxford Nanopore Duplex reads, called using the dorado basecaller are typically stored in SAM/BAM format. After changing to a 16-bit internal quality representation, the reported base quality limit is q50 (S). [8]
The SAM format consists of a header and an alignment section. [1] The binary equivalent of a SAM file is a Binary Alignment Map (BAM) file, which stores the same data in a compressed binary representation. [4] SAM files can be analysed and edited with the software SAMtools. [1] The header section must be prior to the alignment section if it is ...
In Windows 7 and later, significant hardware changes (e.g. motherboard) may require a re-activation. In Windows 10 and 11, a user can run the Activation Troubleshooter if the user has changed hardware on their device recently. If the hardware has changed again after activation, they must wait 30 days before running the troubleshooter again.
Compressed Reference-oriented Alignment Map (CRAM) is a compressed columnar file format for storing biological sequences aligned to a reference sequence, initially devised by Markus Hsi-Yang Fritz et al. [1] CRAM was designed to be an efficient reference-based alternative to the Sequence Alignment Map (SAM) and Binary Alignment Map (BAM) file ...
HTML Form format HTML 4.01 Specification since PDF 1.5; HTML 2.0 since 1.2 Forms Data Format (FDF) based on PDF, uses the same syntax and has essentially the same file structure, but is much simpler than PDF since the body of an FDF document consists of only one required object. Forms Data Format is defined in the PDF specification (since PDF 1.2).
Product activation is a license validation procedure required by some proprietary software programs. Product activation prevents unlimited free use of copied or replicated software. Unactivated software refuses to fully function until it determines whether it is authorized to fully function. Activation allows the software to stop blocking its use.