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Antonie van Leeuwenhoek (1632–1723). The field of microscopy (optical microscopy) dates back to at least the 17th-century.Earlier microscopes, single lens magnifying glasses with limited magnification, date at least as far back as the wide spread use of lenses in eyeglasses in the 13th century [2] but more advanced compound microscopes first appeared in Europe around 1620 [3] [4] The ...
A digital microscope is a variation of a traditional optical microscope that uses optics and a digital camera to output an image to a monitor, sometimes by means of software running on a computer. A digital microscope often has its own in-built LED light source, and differs from an optical microscope in that there is no provision to observe the ...
Diagram of a simple microscope. There are two basic types of optical microscopes: simple microscopes and compound microscopes. A simple microscope uses the optical power of a single lens or group of lenses for magnification. A compound microscope uses a system of lenses (one set enlarging the image produced by another) to achieve a much higher ...
Computational microscopy is a subfield of computational imaging, which combines algorithmic reconstruction with sensing to capture microscopic images of objects. [1] [2] The algorithms used in computational microscopy often combine the information of several images captured using various illuminations or measurements to form an aggregated 2D or 3D image using iterative techniques or machine ...
Digital holography comes in different flavors, such as off-axis Fresnel, Fourier, image plane, in-line, Gabor and phase-shifting digital holography, [2] depending on the optical setup. The basic principle, however, is the same; a hologram is recorded and an image is reconstructed by a computer.
The use of virtual microscopes can transform traditional teaching methods by removing the reliance on physical space, equipment, and specimens to a model that is solely dependent upon computer-internet access. This increases the convenience of accessing the slide sets and making the slides available to a broader audience.
Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
The concept of virtual microscopy emerged in the 1990s across various areas of life science research. [4] At the turn of the century the scientific community more and more agreed on the term "digital pathology" to denote digitization efforts in pathology. However, in 2000, the technical requirements (scanner, storage, network) were still a ...
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