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The count represents the number of colony forming units (cfu) per g (or per ml) of the sample. A TVC is achieved by plating serial tenfold dilutions of the sample until between 30 and 300 colonies can be counted on a single plate. The reported count is the number of colonies counted multiplied by the dilution used for the counted plate
Determining the viable cell count is important for calculating dilutions required for the passaging of cells, as well as determining the size and number of flasks needed during growth time. It is also vital when seeding plates for assays, such as the plaque assay , [ 2 ] because the plates need a known number of live replicating cells for the ...
Once a plate has been successfully prepared, plate count agar cells will grow into colonies which can be sufficiently isolated to determine the original cell type. The colony-forming unit (CFU) is an appropriate description of the colony's origin. In plate counts, colonies are counted, but the count is usually recorded in CFU.
NICE is a program written in MATLAB that provides an easy way to count colonies from images. [18] ImageJ and CellProfiler: Some ImageJ macros [19] and plugins and some CellProfiler pipelines [20] can be used to count colonies. This often requires the user to change the code in order to achieve an efficient work-flow, but can prove useful and ...
Graphical examination of count data may be aided by the use of data transformations chosen to have the property of stabilising the sample variance. In particular, the square root transformation might be used when data can be approximated by a Poisson distribution (although other transformation have modestly improved properties), while an inverse sine transformation is available when a binomial ...
Others noted how people who count on these ceremonies to make a living could suffer in the process. “Award season provides essential work for so many — makeup artists, drivers, stylists ...
The simplest method for correcting the reticulocyte count, to obtain a more accurate daily production index, is to divide the corrected count by a factor of 2 (or multiply with ½) whenever polychromasia (the presence of immature marrow reticulocytes or "shift" cells) is observed on the smear or the immature fraction on the automated counter is ...
For data in which the maximum key size is significantly smaller than the number of data items, counting sort may be parallelized by splitting the input into subarrays of approximately equal size, processing each subarray in parallel to generate a separate count array for each subarray, and then merging the count arrays.