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Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.2 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts.
One of the most important properties of microscope objectives is their magnification. The magnification typically ranges from 4× to 100×. It is combined with the magnification of the eyepiece to determine the overall magnification of the microscope; a 4× objective with a 10× eyepiece produces an image that is 40 times the size of the object.
In microscopy, the field of view in high power (usually a 400-fold magnification when referenced in scientific papers) is called a high-power field, and is used as a reference point for various classification schemes. For an objective with magnification , the FOV is related to the Field Number (FN) by
The area provides a reference unit, for example in reference ranges for urine tests. [3]Used for grading of soft tissue tumors: Grading, usually on a scale of I to III, is based on the degree of differentiation, the average number of mitoses per high-power field, cellularity, pleomorphism, and an estimate of the extent of necrosis (presumably a reflection of rate of growth).
Resolving power is the ability of an imaging device to separate (i.e., to see as distinct) points of an object that are located at a small angular distance or it is the power of an optical instrument to separate far away objects, that are close together, into individual images.
Of particular relevance for low-light and astronomical viewing is the ratio between magnifying power and objective lens diameter. A lower magnification facilitates a larger field of view which is useful in viewing the Milky Way and large nebulous objects (referred to as deep sky objects) such as the nebulae and galaxies. The large (typical 7.14 ...
In optics, optical power (also referred to as dioptric power, refractive power, focusing power, or convergence power) is the degree to which a lens, mirror, or other optical system converges or diverges light. It is equal to the reciprocal of the focal length of the device: P = 1/f. [1] High optical power corresponds to short focal length.
A 1951 USAF resolution test chart is a microscopic optical resolution test device originally defined by the U.S. Air Force MIL-STD-150A standard of 1951. The design provides numerous small target shapes exhibiting a stepped assortment of precise spatial frequency specimens.
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