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Two Leica oil immersion microscope objective lenses; left 100×, right 40×. The objective lens of a microscope is the one at the bottom near the sample. At its simplest, it is a very high-powered magnifying glass, with very short focal length. This is brought very close to the specimen being examined so that the light from the specimen comes ...
Oil immersion objective lenses look superficially identical to non-oil immersion lenses. In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical ...
This definition of lens power relies upon an arbitrary decision to split the angular magnification of the instrument into separate factors for the eyepiece and the objective. Historically, Abbe described microscope eyepieces differently, in terms of angular magnification of the eyepiece and 'initial magnification' of the objective.
The refractive index of the index-matching material is higher than air allowing the objective lens to have a larger numerical aperture (greater than 1) so that the light is transmitted from the specimen to the outer face of the objective lens with minimal refraction. Numerical apertures as high as 1.6 can be achieved. [26]
A high power objective lens is typically used. This both maximises the solid angle subtended by the lens, and hence the angular variation of the light intercepted, and also increases the likelihood that only a single crystal will be viewed at any given time.
By virtue of the linearity property of optical non-coherent imaging systems, i.e., . Image(Object 1 + Object 2) = Image(Object 1) + Image(Object 2). the image of an object in a microscope or telescope as a non-coherent imaging system can be computed by expressing the object-plane field as a weighted sum of 2D impulse functions, and then expressing the image plane field as a weighted sum of the ...
In light microscopy, a water immersion objective is a specially designed objective lens used to increase the resolution of the microscope. This is achieved by immersing both the lens and the specimen in water which has a higher refractive index than air, thereby increasing the numerical aperture of the objective lens.
In microscopy, the field of view in high power (usually a 400-fold magnification when referenced in scientific papers) is called a high-power field, and is used as a reference point for various classification schemes. For an objective with magnification , the FOV is related to the Field Number (FN) by