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MCF-7 Cells. MCF-7 is a breast cancer cell line isolated in 1970 from a 69-year-old White woman. [1] MCF-7 is the acronym of Michigan Cancer Foundation-7, referring to the institute in Detroit where the cell line was established in 1973 by Herbert Soule and co-workers. [2]
Scientists study the behaviour of isolated cells grown in the laboratory for insights into how cells function in the body in health and disease. Experiments using cell culture are used for developing new diagnostic tests and new treatments for diseases. This is a list of major breast cancer cell lines that are primarily used in breast cancer ...
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Map of the United States with Pennsylvania highlighted. There are 56 municipalities classified as cities in the U.S. state of Pennsylvania. [1] Each city is further classified based on population, with Philadelphia being of the first class, Pittsburgh of the second class, Scranton of the second class A, and the remaining 53 cities being of the third class.
The E-SCREEN cell proliferation assay is performed with the human MCF-7 breast cancer cell line, an established estrogenic cell line that endogenously expresses ERα. [ 1 ] [ 3 ] [ 4 ] Human MCF-7 are cultivated in Dulbecco’s modified Eagle’s medium (DMEM) with fetal bovine serum (FBS) and phenol red as buffer tracer (culture medium), at 37 ...
Map of the United States with Pennsylvania highlighted in red. Pennsylvania is a state located in the Northeastern United States. As of the 2020 U.S. census, Pennsylvania is the fifth-most populous state with 13,002,700 inhabitants [1] and the 32nd-largest by land area spanning 44,742.70 square miles (115,883.1 km 2) of land. [2]
A 61st cell line, MDA-N, has been confirmed to being derived from the misclassified MDA-MB-435 cell line. [7] So it is also misclassified, really being a melanoma cell line, but as of 6 January 2018 the official website still lists it as breast cancer cell line. [1] This cell line is not available as of 6 January 2018. [1]
There are two principal ways in which a cell line can become contaminated: cell cultures are often exchanged between research groups; if, during handling, a sample is contaminated and then passed on, subsequent exchanges of cells will lead to the contaminating population being established, although parts of the supposed cell line are still genuine.