enow.com Web Search

Search results

  1. Results from the WOW.Com Content Network
  2. DNA replication - Wikipedia

    en.wikipedia.org/wiki/DNA_replication

    Eukaryotes initiate DNA replication at multiple points in the chromosome, so replication forks meet and terminate at many points in the chromosome. Because eukaryotes have linear chromosomes, DNA replication is unable to reach the very end of the chromosomes. Due to this problem, DNA is lost in each replication cycle from the end of the chromosome.

  3. DNA synthesis - Wikipedia

    en.wikipedia.org/wiki/DNA_synthesis

    Control of the DNA replication system ensures that the genome is replicated only once per cycle; over-replication induces DNA damage. Deregulation of DNA replication is a key factor in genomic instability during cancer development. [3] This highlights the specificity of DNA synthesis machinery in vivo. Various means exist to artificially ...

  4. Polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Polymerase_chain_reaction

    A strip of eight PCR tubes, each containing a 100 μL reaction mixture Placing a strip of eight PCR tubes into a thermal cycler. The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study.

  5. Overlap extension polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Overlap_extension...

    The duplex is denatured again and the first primer can now bind to the latest DNA strand. The replication reaction continues to produce a fully dimerised DNA fragment. After further PCR cycles, to amplify the DNA, the sample can be separated by agarose gel electrophoresis, followed by electroelution for collection.

  6. Rolling circle replication - Wikipedia

    en.wikipedia.org/wiki/Rolling_circle_replication

    Rolling circle replication (RCR) is a process of unidirectional nucleic acid replication that can rapidly synthesize multiple copies of circular molecules of DNA or RNA, such as plasmids, the genomes of bacteriophages, and the circular RNA genome of viroids. Some eukaryotic viruses also replicate their DNA or RNA via the rolling circle mechanism.

  7. Replication timing - Wikipedia

    en.wikipedia.org/wiki/Replication_timing

    Figure 1: Schematic of the cell cycle. outer ring: I = Interphase, M = Mitosis; inner ring: M = Mitosis, G 1 = Gap 1, G 2 = Gap 2, S = Synthesis; not in ring: G 0 = Gap 0/Resting. Replication timing refers to the order in which segments of DNA along the length of a chromosome are duplicated.

  8. DNA polymerase - Wikipedia

    en.wikipedia.org/wiki/DNA_polymerase

    A DNA polymerase is a member of a family of enzymes that catalyze the synthesis of DNA molecules from nucleoside triphosphates, the molecular precursors of DNA.These enzymes are essential for DNA replication and usually work in groups to create two identical DNA duplexes from a single original DNA duplex.

  9. Replisome - Wikipedia

    en.wikipedia.org/wiki/Replisome

    DNA is a duplex formed by two anti-parallel strands. Following Meselson-Stahl, the process of DNA replication is semi-conservative, whereby during replication the original DNA duplex is separated into two daughter strands (referred to as the leading and lagging strand templates). Each daughter strand becomes part of a new DNA duplex.