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A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used ...
A strip of eight PCR tubes, each containing a 100 μL reaction mixture Placing a strip of eight PCR tubes into a thermal cycler. The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR). [1] It is primarily used to measure the amount of a specific RNA.
Acid Chloride Preparative Route for Nylon-6,10, which is often used in the nylon rope trick. The nylon rope trick is a scientific demonstration that illustrates some of the fundamental chemical principles of step-growth polymerization and provides students and other observers with a hands-on demonstration of the preparation of a synthetic polymer.
Digital polymerase chain reaction (digital PCR, DigitalPCR, dPCR, or dePCR) is a biotechnological refinement of conventional polymerase chain reaction methods that can be used to directly quantify and clonally amplify nucleic acids strands including DNA, cDNA, or RNA.
As stated above on this page, "The term 'real-time PC' and its variant 'qPCR' are very widely and interchangeably used in molecular biology research". Many people consider these two terms as synonymous. And real-time PCR is abbreviated qPCR so any distinction tend to disappear. The article should use the most common, most logical and most used ...
In chain-growth (or chain) polymerization, the only chain-extension reaction step is the addition of a monomer to a growing chain with an active center such as a free radical, cation, or anion. Once the growth of a chain is initiated by formation of an active center, chain propagation is usually rapid by addition of a sequence of monomers.
Single polymer molecules (0.4 nm thick chains) recorded under aqueous media at different pH using an AFM. Drastic change of polymer chain conformation is observed at a small change of pH. [1] A single-molecule experiment is an experiment that investigates the properties of individual molecules.