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  2. Chromatography - Wikipedia

    en.wikipedia.org/wiki/Chromatography

    Thin-layer chromatography is used to separate components of a plant extract, illustrating the experiment with plant pigments which gave chromatography its name. In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components.

  3. Liquid–liquid extraction - Wikipedia

    en.wikipedia.org/wiki/Liquid–liquid_extraction

    The separation factor is one distribution ratio divided by another; it is a measure of the ability of the system to separate two solutes. For instance, if the distribution ratio for nickel (D Ni) is 10 and the distribution ratio for silver (D Ag) is 100, then the silver/nickel separation factor (SF Ag/Ni) is equal to D Ag /D Ni = SF Ag/Ni = 10.

  4. High-performance liquid chromatography - Wikipedia

    en.wikipedia.org/wiki/High-performance_liquid...

    Separation factor (alpha) is a relative comparison on how well two neighboring components of the mixture were separated (i.e., two neighboring bands on a chromatogram). This factor is defined in terms of a ratio of the retention factors of a pair of neighboring chromatogram peaks, and may also be corrected for by the void volume of the column.

  5. Resolution (chromatography) - Wikipedia

    en.wikipedia.org/wiki/Resolution_(chromatography)

    Resolution (chromatography) ... In chromatography, resolution is a measure of the separation of two peaks of different retention time t in a chromatogram. [1] [2] [3] [4]

  6. Reversed-phase chromatography - Wikipedia

    en.wikipedia.org/wiki/Reversed-phase_chromatography

    Reversed-phase liquid chromatography (RP-LC) is a mode of liquid chromatography in which non-polar stationary phase and polar mobile phases are used for the separation of organic compounds. [ 1 ] [ 2 ] [ 3 ] The vast majority of separations and analyses using high-performance liquid chromatography (HPLC) in recent years are done using the ...

  7. Paper chromatography - Wikipedia

    en.wikipedia.org/wiki/Paper_chromatography

    Paper chromatography is a useful technique because it is relatively quick and requires only small quantities of material. Separations in paper chromatography involve the principle of partition. In paper chromatography, substances are distributed between a stationary phase and a mobile phase.

  8. Distribution constant - Wikipedia

    en.wikipedia.org/wiki/Distribution_constant

    The distribution constant (or partition ratio) (K D) is the equilibrium constant for the distribution of an analyte in two immiscible solvents. [1] [2] [3]In chromatography, for a particular solvent, it is equal to the ratio of its molar concentration in the stationary phase to its molar concentration in the mobile phase, also approximating the ratio of the solubility of the solvent in each phase.

  9. Affinity chromatography - Wikipedia

    en.wikipedia.org/wiki/Affinity_chromatography

    Weak affinity chromatography [29] (WAC) is an affinity chromatography technique for affinity screening in drug development. [ 30 ] [ 31 ] WAC is an affinity-based liquid chromatographic technique that separates chemical compounds based on their different weak affinities to an immobilized target.

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