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Indole test positive: appearance of pink layer at top (e.g. Escherichia coli) Like many biochemical tests on bacteria, results of an indole test are indicated by a change in color following a reaction with an added reagent. Pure bacterial culture must be grown in sterile tryptophan or peptone broth for 24–48 hours before performing the test.
The species C. amalonaticus, C. koseri, and C. freundii can use citrate as a sole carbon source. Citrobacter species are differentiated by their ability to convert tryptophan to indole (C. koseri is the only citrobacter to be commonly indole-positive), ferment lactose (C. koseri is a lactose fermentor), and use malonate.
The IMViC tests are a group of individual tests used in microbiology lab testing to identify an organism in the coliform group.A coliform is a gram negative, aerobic, or facultative anaerobic rod, which produces gas from lactose within 48 hours.
An indole test will differentiate Enterobacter from Escherichia, as Enterobacter are indole negative and Escherichia is positive. [5] Enterobacter are distinguished from Klebsiella because of their differences in motility. [5] Klebsiella are non-motile, Gram-negative bacilli ranging from 1–2 μm in length. [6]
A new species within the genus Peptostreptococcus is Peptostreptococcus hydrogenalis; it contains the indole-positive, saccharolytic strains of the genus. [10] Peptostreptococcus infections occur in/on all body sites, including the CNS, head, neck, chest, abdomen, pelvis, skin, bone, joint, and soft tissues. Adequate therapy must be taken ...
Proteus vulgaris is a rod-shaped, nitrate-reducing, indole-positive and catalase-positive, hydrogen sulfide-producing, Gram-negative bacterium that inhabits the intestinal tracts of humans and animals. It can be found in soil, water, and fecal matter.
Proteus is a genus of Gram-negative bacteria. It is a rod shaped, aerobic and motile bacteria, which is able to migrate across surfaces due its “swarming” characteristic in temperatures between 20 and 37 °C. [1] Their size generally ranges from 0.4 to 0.8 μm in diameter and 1.0–3.0 μm in length. They tend to have an ammonia smell. [2]
P. hauseri is ONPG negative and PDA positive [citation needed]. Unlike the more commonly seen species of Proteus, P. hauseri is also able to convert tryptophan into indole, resulting in a positive indole test. P. hauseri shares a similar biochemical profile with Proteus vulgaris but can be differentiated by its ability to produce acid from ...