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  2. In silico PCR - Wikipedia

    en.wikipedia.org/wiki/In_silico_PCR

    The last 10-12 bases at the 3' end of a primer are sensitive to initiation of polymerase extension and general primer stability on the template binding site. The effect of a single mismatch at these last 10 bases at the 3' end of the primer depends on its position and local structure, reducing the primer binding, selectivity, and PCR efficiency.

  3. Primer (molecular biology) - Wikipedia

    en.wikipedia.org/wiki/Primer_(molecular_biology)

    Additionally, primer sequences need to be chosen to uniquely select for a region of DNA, avoiding the possibility of hybridization to a similar sequence nearby. A commonly used method for selecting a primer site is BLAST search, whereby all the possible regions to which a primer may bind can be seen. Both the nucleotide sequence as well as the ...

  4. Amplified fragment length polymorphism - Wikipedia

    en.wikipedia.org/wiki/Amplified_fragment_length...

    Amplified fragment length polymorphism (AFLP-PCR or AFLP) is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. Developed in the early 1990s by KeyGene , [ 1 ] AFLP uses restriction enzymes to digest genomic DNA , followed by ligation of adaptors to the sticky ends of the restriction ...

  5. Random amplification of polymorphic DNA - Wikipedia

    en.wikipedia.org/wiki/Random_amplification_of...

    Random amplified polymorphic DNA (RAPD), pronounced "rapid", [1] is a type of polymerase chain reaction (PCR), but the segments of DNA that are amplified are random. [2] The scientist performing RAPD creates several arbitrary, short primers (10–12 nucleotides), then proceeds with the PCR using a large template of genomic DNA, hoping that fragments will amplify.

  6. Primer dimer - Wikipedia

    en.wikipedia.org/wiki/Primer_dimer

    A primer dimer (PD) is a potential by-product in the polymerase chain reaction (PCR), a common biotechnological method. As its name implies, a PD consists of two primer molecules that have attached to each other because of strings of complementary bases in the primers.

  7. Fantasy football Start 'Em, Sit 'Em: Players to start or sit ...

    www.aol.com/fantasy-football-start-em-sit...

    Four games are in the books for Week 13 but there's still a lot of action to go in fantasy football. Here are players to start or sit this week.

  8. France government collapses again after prime minister forced out

    www.aol.com/news/france-government-collapses...

    Here's a brief primer on the issue. Why is this happening now? Trouble has been brewing since Barnier, a conservative, was asked by Macron, a centrist, ...

  9. Hot start PCR - Wikipedia

    en.wikipedia.org/wiki/Hot_start_PCR

    For example, oligonucleotides with a hairpin structure cannot act efficiently as a primer. However, after heating the reaction mix to the annealing temperature the primer will undergo a conformation change allowing the primer to form a linear structure instead, which enables the primer to attach to the target segment and begin PCR.