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Additionally, primer sequences need to be chosen to uniquely select for a region of DNA, avoiding the possibility of hybridization to a similar sequence nearby. A commonly used method for selecting a primer site is BLAST search, whereby all the possible regions to which a primer may bind can be seen. Both the nucleotide sequence as well as the ...
The last 10-12 bases at the 3' end of a primer are sensitive to initiation of polymerase extension and general primer stability on the template binding site. The effect of a single mismatch at these last 10 bases at the 3' end of the primer depends on its position and local structure, reducing the primer binding, selectivity, and PCR efficiency.
In chemistry, a chemical test is qualitative or semi-quantitative procedure designed to prove the existence of a chemical compound or chemical group with the aid of a specific reagent. Subcategories This category has the following 3 subcategories, out of 3 total.
A list of chemical analysis methods with acronyms. A. Atomic absorption spectroscopy (AAS) Atomic emission spectroscopy (AES) Atomic fluorescence spectroscopy (AFS) ...
At least one of the primers is chosen from a polymorphic area, with the mutations located at (or near) its 3'-end. Under stringent conditions, a mismatched primer will not initiate replication, whereas a matched primer will. The appearance of an amplification product therefore indicates the genotype. (For more information, see SNP genotyping.)
Take k=3 for example, we list the words of length 3 in the query protein sequence (k is usually 11 for a DNA sequence) "sequentially", until the last letter of the query sequence is included. The method is illustrated in figure 1. Fig. 1 The method to establish the k-letter query word list. [14] List the possible matching words.
Immunohistochemistry test; Immunomagnetic separation; Immunoperoxidase; Immunosequencing; Impalefection; In situ hybridization; In vitro; Indirect immunoperoxidase assay; Induced cell cycle arrest; Inert salt; Intravital microscopy; Inverse polymerase chain reaction; Iodine–starch test; Ion-mobility spectrometry–mass spectrometry; Ion ...
In a laboratory setting, mixture of dissolved materials are typically fed using a solvent into a column packed with an appropriate adsorbent, and due to different affinities for solvent (moving phase) versus adsorbent (stationary phase) the components in the original mixture pass through the column in the moving phase at different rates, which ...