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The excess-velocity blast-yield measurement (precise measurement of the velocity of sound at the site of the explosion and then comparing it with the velocity of the blast wave) [128] provided among the most accurate measurements of the blast pressure. Another method was to use the aluminum diaphragm box gauges designed to record the peak ...
The free NCBI tool Primer-BLAST integrates primer design and BLAST search into one application, [6] as do commercial software products such as ePrime and Beacon Designer. Computer simulations of theoretical PCR results ( Electronic PCR ) may be performed to assist in primer design by giving melting and annealing temperatures, etc. [ 7 ]
The last 10-12 bases at the 3' end of a primer are sensitive to initiation of polymerase extension and general primer stability on the template binding site. The effect of a single mismatch at these last 10 bases at the 3' end of the primer depends on its position and local structure, reducing the primer binding, selectivity, and PCR efficiency.
Prediabetes is almost always a precursor to type 2 diabetes — but it doesn’t have to lead to that outcome. Experts share healthy steps to reverse the condition.
A primer dimer (PD) is a potential by-product in the polymerase chain reaction (PCR), a common biotechnological method. As its name implies, a PD consists of two primer molecules that have attached to each other because of strings of complementary bases in the primers.
Jennifer Lopes was one of the many, many, mannnny celebrities to show up at the 15th annual Governors Awards last night, and truly turned it out for the red carpet.
The early morning killing of a top health insurance executive in midtown Manhattan Wednesday has unleashed a flurry of rage and frustration from social media users over denials of their medical ...
For example, oligonucleotides with a hairpin structure cannot act efficiently as a primer. However, after heating the reaction mix to the annealing temperature the primer will undergo a conformation change allowing the primer to form a linear structure instead, which enables the primer to attach to the target segment and begin PCR.