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BCA protein assay in a 96 well plate. The bicinchoninic acid assay (BCA assay), also known as the Smith assay, after its inventor, Paul K. Smith at the Pierce Chemical Company, [1] now part of Thermo Fisher Scientific, is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL), similar to Lowry protein assay, Bradford protein assay or ...
The first industrial process involved the reaction of benzotrichloride (trichloromethyl benzene) with calcium hydroxide in water, using iron or iron salts as catalyst. The resulting calcium benzoate is converted to benzoic acid with hydrochloric acid. The product contains significant amounts of chlorinated benzoic acid derivatives.
The pH range is commonly given as zero to 14, but a pH value can be less than 0 for very concentrated strong acids or greater than 14 for very concentrated strong bases. [2] The pH scale is traceable to a set of standard solutions whose pH is established by international agreement. [3]
Heroin users are known to use ascorbic acid as a means to convert heroin base to a water-soluble salt so that it can be injected. [21] As justified by its reaction with iodine, it is used to negate the effects of iodine tablets in water purification. It reacts with the sterilized water, removing the taste, color, and smell of the iodine.
ISO/IEC 15504 is the reference model for the maturity models (consisting of capability levels which in turn consist of the process attributes and further consist of generic practices) against which the assessors can place the evidence that they collect during their assessment, so that the assessors can give an overall determination of the organization's capabilities for delivering products ...
In aqueous solution at pH close to neutrality, amino acids exist as zwitterions, i.e. as dipolar ions with both NH + 3 and CO − 2 in charged states, so the overall structure is NH + 3 −CHR−CO − 2. At physiological pH the so-called "neutral forms" −NH 2 −CHR−CO 2 H are not present to any measurable degree. [36]
The inverse electron demand Diels–Alder reaction, or DA INV or IEDDA [1] is an organic chemical reaction, in which two new chemical bonds and a six-membered ring are formed. It is related to the Diels–Alder reaction , but unlike the Diels–Alder (or DA) reaction, the DA INV is a cycloaddition between an electron-rich dienophile and an ...
Gareth Frank Bale MBE (born 16 July 1989) is a Welsh former professional footballer who played as a right winger, most notably for Tottenham Hotspur, Real Madrid, and the Wales national team.