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2. Illustration of electrophoresis retardation. Electrophoresis is the motion of charged dispersed particles or dissolved charged molecules relative to a fluid under the influence of a spatially uniform electric field. As a rule, these are zwitterions. [1] Electrophoresis is used in laboratories to separate macromolecules based on their
The moving-boundary electrophoresis apparatus includes a U-shaped cell filled with buffer solution and electrodes immersed at its ends. The sample applied could be any mixture of charged components such as a protein mixture. On applying voltage, the compounds will migrate to the anode or cathode depending on their charges.
Electrophoresis is a method of moving charged particles through a medium by using an electric field induced by electrodes. It is also used to separate molecules with different physical characteristics using electrical charges.
Isotachophoresis (ITP) is a technique in analytical chemistry used for selective separation and concentration of ionic analytes. It is a form of electrophoresis; charged analytes are separated based on ionic mobility, a quantity which tells how fast an ion migrates through an electric field.
Electrochromatography is a chemical separation technique in analytical chemistry, biochemistry and molecular biology used to resolve and separate mostly large biomolecules such as proteins. It is a combination of size exclusion chromatography (gel filtration chromatography) and gel electrophoresis. These separation mechanisms operate ...
Microelectrophoresis is a method of studying electrophoresis of various dispersed particles using optical microscopy. This method provides image of moving particles, which is its unique advantage. e.g. observation of RBCs, neutrophiles and bacteria.
An electropherogram (also called electrophoretogram, sequencing chromatogram, EPG, and e-gram) is a record or chart produced when electrophoresis is used in an analytical technique, primarily in the fields of forensic biology, molecular biology, and biochemistry. [1]
electrophoresis, as motion of charged particles under influence of electric field; electro-osmosis, as motion of liquid in porous body under influence of electric field; diffusiophoresis, as motion of particles under influence of a chemical potential gradient;