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The blue–white screen is a screening technique that allows for the rapid and convenient detection of recombinant bacteria in vector-based molecular cloning experiments. This method of screening is usually performed using a suitable bacterial strain , but other organisms such as yeast may also be used.
This design enables efficient identification of recombinant plasmids, as the insertion of foreign DNA into the MCS disrupts the marker gene, allowing for blue-white screening or other selection methods. [6] In expression vectors, MCSs are positioned between a promoter and a terminator to regulate gene expression. The upstream promoter can be ...
This allows for blue–white screening when using host strains such as E. coli JM109, which produces only the C-terminal portion of lacZ, also known as the β-polypeptide. [3] If pUC19 is inserted into E. coli JM109 and grown on agar media supplemented with IPTG and X-gal , then colonies will appear blue, as the plasmid encodes for the α ...
An alternative to a selectable marker is a screenable marker, another type of reporter gene which allows the researcher to distinguish between wanted and unwanted cells or colonies, such as between blue and white colonies in blue–white screening. These wanted or unwanted cells are simply non-transformed cells that were unable to take up the ...
Such features present in cloning vectors may be the lacZα fragment for α complementation in blue-white selection, and/or marker gene or reporter genes in frame with and flanking the MCS to facilitate the production of fusion proteins. Examples of fusion partners that may be used for screening are the green fluorescent protein (GFP) and ...
Red-blue party division, explained. Olivia Munson, USA TODAY. Updated November 5, 2024 at 4:33 PM. The 2024 election is today, and the results will usher in the 119th Congress.
White Elephant gift exchanges are common during the holidays. Here's what to know about the rules of the exchange and how to play.
A test that identifies biomarkers associated with autism just became available in most states. It's meant to help rule out autism in children who have higher likelihoods of it.