Search results
Results from the WOW.Com Content Network
Protein production is the biotechnological process of generating a specific protein. It is typically achieved by the manipulation of gene expression in an organism such that it expresses large amounts of a recombinant gene.
Recombinant proteins are produced through a step-by-step procedure involving gene isolation, DNA cloning, transformation, selection, protein expression, purification, quality control and scale-up production.
As a tool, recombinant DNA technology can be used to express proteins towards medical applications. Prior to biotechnology, type I diabetes (insulin-dependent) was treated by injection of insulin isolated from the pancreas of pigs.
In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus...
As alluded to earlier, the process of producing recombinant proteins is highly dependent on the process of inserting the DNA segment to the host’s genome. Two methods are known to produce recombinant DNA namely molecular cloning and PCR. 1. Through Molecular Cloning.
Overexpression of recombinant protein often leads to severe burden on the physiology of yeast and triggers cellular stress. Yu et al. identified novel factors to enhance recombinant protein production in multi-copy K. phaffii based on transcriptomic analysis of overexpression effects.
We experimentally validate many of the predicted targets for α-amylase production to demonstrate pcSecYeast application as a computational tool in guiding yeast engineering and improving...