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  2. Spin column-based nucleic acid purification - Wikipedia

    en.wikipedia.org/wiki/Spin_column-based_nucleic...

    The different stages of the method are lyse, bind, wash, and elute. [1] [2] More specifically, this entails the lysis of target cells to release nucleic acids, selective binding of nucleic acid to a silica membrane, washing away particulates and inhibitors that are not bound to the silica membrane, and elution of the nucleic acid, with the end result being purified nucleic acid in an aqueous ...

  3. Plasmid preparation - Wikipedia

    en.wikipedia.org/wiki/Plasmid_preparation

    Plasmid miniprep. 0.8% agarose gel ethidium bromide-stained.. A plasmid preparation is a method of DNA extraction and purification for plasmid DNA.It is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology.

  4. Qiagen - Wikipedia

    en.wikipedia.org/wiki/Qiagen

    In 2019, QIAGEN announced the acquisition of Formulatrix assets to develop a digital PCR platform. [28] An additional acquisition of N-of-One, a privately held U.S. molecular decision support company and pioneer in clinical interpretation services for complex genomic data, allowed it to expand its Qiagen Clinical Insights (QCI) bioinformatics ...

  5. DNA extraction - Wikipedia

    en.wikipedia.org/wiki/DNA_extraction

    For the chemical method, many different kits are used for extraction, and selecting the correct one will save time on kit optimization and extraction procedures. PCR sensitivity detection is considered to show the variation between the commercial kits. [5] There are many different methods for extracting DNA, but some common steps include:

  6. His-tag - Wikipedia

    en.wikipedia.org/wiki/His-tag

    The tag was invented by Roche, [1] although the use of histidines and its vectors are distributed by Qiagen. Various purification kits for histidine-tagged proteins are commercially available from multiple companies. [2] The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues.

  7. Buffer P2 - Wikipedia

    en.wikipedia.org/wiki/Buffer_P2

    Buffer P2 is a lysis buffer solution produced by Qiagen.It contains 1% sodium dodecyl sulfate (SDS) (w/v) to puncture holes in cellular membranes, and 200mM NaOH.It is used in conjunction with other resuspension buffers and lysis buffers to release DNA from cells, often as part of the alkaline lysis method of purifying plasmid DNA from bacterial cell culture.

  8. Thermal cycler - Wikipedia

    en.wikipedia.org/wiki/Thermal_cycler

    The thermal cycler (also known as a thermocycler, PCR machine or DNA amplifier) is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR). [1] Thermal cyclers may also be used in laboratories to facilitate other temperature-sensitive reactions, including restriction enzyme digestion or rapid ...

  9. Lysis buffer - Wikipedia

    en.wikipedia.org/wiki/Lysis_buffer

    Cell lysis is a critical step in the purification of enzymes from bacterial cells, various components are commonly included in lysing buffers to facilitate effective cell disruption and release of the target enzyme. These components include detergents, salts, and enzymes, each playing a specific role in the lysis process.