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Mitzi Kuroda is a professor of genetics at Harvard Medical School and Brigham and Women's Hospital. [1] She was an HHMI Investigator at Brigham and Women's Hospital from 1993 to 2007. [2]
HHMI spends about $1 million per HHMI Investigator per year, which amounts to annual investment in biomedical research of about $825 million. The institute has an endowment of $22.6 billion , making it the second-wealthiest philanthropic organization in the United States and the second-best-endowed medical research foundation in the world. [ 6 ]
Since 1995, Berry has been a biomedical animator at the Walter and Eliza Hall Institute of Medical Research. [2] His 3D and 4D animations have focussed on explaining cellular and molecular processes relevant to research conducted at the institute, in fields including molecular biology, malaria, cell death, cancer biology, hematology and immunology.
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Targeted gene knockout using CRISPR/Cas9 requires the use of a delivery system to introduce the sgRNA and Cas9 into the cell. Although a number of different delivery systems are potentially available for CRISPR, [37] [38] genome-wide loss-of-function screens are predominantly carried out using third generation lentiviral vectors.
CRISPR-associated transposons or CASTs are mobile genetic elements that have evolved to make use of minimal CRISPR systems for RNA-guided transposition of their DNA. [1] Unlike traditional CRISPR systems that contain interference mechanisms to degrade targeted DNA, CASTs lack proteins and/or protein domains responsible for DNA cleavage. [ 2 ]
Jennifer Doudna was born February 19, 1964, in Washington, D.C., as the daughter of Dorothy Jane (Williams) and Martin Kirk Doudna. [2] [17] Her father received his PhD in English literature from the University of Michigan, and her mother held a master's degree in education.
Bert Vogelstein (born 1949) is director of the Ludwig Center, Clayton Professor of Oncology and Pathology and a Howard Hughes Medical Institute investigator at The Johns Hopkins Medical School and Sidney Kimmel Comprehensive Cancer Center. [4]