enow.com Web Search

Search results

  1. Results from the WOW.Com Content Network
  2. Φ29 DNA polymerase - Wikipedia

    en.wikipedia.org/wiki/Φ29_DNA_polymerase

    The polymerase is a monomeric protein with two distinct functional domains. Site-directed mutagenesis experiments support the proposition that this protein displays a structural and functional similarity to the Klenow fragment of the Escherichia coli Polymerase I enzyme; [3] it comprises a C-terminal polymerase domain and a spatially separated N-terminal domain with a 3'-5' exonuclease activity.

  3. DNA polymerase - Wikipedia

    en.wikipedia.org/wiki/DNA_polymerase

    A DNA polymerase is a member of a family of enzymes that catalyze the synthesis of DNA molecules from nucleoside triphosphates, the molecular precursors of DNA. These enzymes are essential for DNA replication and usually work in groups to create two identical DNA duplexes from a single original DNA duplex.

  4. Prokaryotic DNA replication - Wikipedia

    en.wikipedia.org/wiki/Prokaryotic_DNA_replication

    Prokaryotic DNA Replication is the process by which a prokaryote duplicates its DNA into another copy that is passed on to daughter cells. [1] Although it is often studied in the model organism E. coli, other bacteria show many similarities. [2] Replication is bi-directional and originates at a single origin of replication (OriC). [3]

  5. Eukaryotic DNA replication - Wikipedia

    en.wikipedia.org/wiki/Eukaryotic_DNA_replication

    Polymerase ε synthesizes DNA on the "leading" DNA strand continuously as it is pointing in the same direction as DNA unwinding by the replisome. In contrast, polymerase δ synthesizes DNA on the "lagging" strand, which is the opposite DNA template strand, in a fragmented or discontinuous manner.

  6. Postreplication repair - Wikipedia

    en.wikipedia.org/wiki/Postreplication_repair

    Incomplete DNA synthesis and DNA strand breaks are both potential sources of genomic instability. An arsenal of DNA repair mechanisms exists to repair various forms of damaged DNA and minimize genomic instability. Most DNA repair mechanisms require an intact DNA strand as template to fix the damaged strand.

  7. DNA polymerase alpha - Wikipedia

    en.wikipedia.org/wiki/Polymerase_(DNA_directed...

    DNA polymerase alpha, like DNA primase, contains iron-sulfur clusters, that are critical in electron transport that uses DNA itself to transfer electrons at very high speeds; this process is involved in detecting DNA damage, and may also be involved in a feedback between the primase complex and the DNA polymerase alpha.

  8. Single-molecule real-time sequencing - Wikipedia

    en.wikipedia.org/wiki/Single-molecule_real-time...

    The DNA sequencing is done on a chip that contains many ZMWs. Inside each ZMW, a single active DNA polymerase with a single molecule of single stranded DNA template is immobilized to the bottom through which light can penetrate and create a visualization chamber that allows monitoring of the activity of the DNA polymerase at a single molecule level.

  9. DNA polymerase I - Wikipedia

    en.wikipedia.org/wiki/DNA_polymerase_I

    DNA polymerase I (or Pol I) is an enzyme that participates in the process of prokaryotic DNA replication. Discovered by Arthur Kornberg in 1956, [1] it was the first known DNA polymerase (and the first known of any kind of polymerase). It was initially characterized in E. coli and is ubiquitous in prokaryotes.