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In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens. Without oil, light waves reflect off the slide specimen ...
The index of the oil is typically chosen to match the index of the microscope lens glass, and of the cover slip. For more details, see the main article, oil immersion. Some microscopes also use other index-matching materials besides oil; see water immersion objective and solid immersion lens.
Becke line test. The Becke line test is a technique in optical mineralogy that helps determine the relative refractive index of two materials. It is done by lowering the stage (increasing the focal distance) of the petrographic microscope and observing which direction the light appears to move. This movement will always go into the material of ...
Bright-field microscopy (BF) is the simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light, and contrast in the sample is caused by attenuation of the transmitted light in dense areas of the sample.
In optics, the refractive index (or refraction index) of an optical medium is a dimensionless number that gives the indication of the light bending ability of that medium. Refraction of a light ray. The refractive index determines how much the path of light is bent, or refracted, when entering a material. This is described by Snell's law of ...
Optical microscope. The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.
In optical microscopes a darkfield condenser lens must be used, which directs a cone of light away from the objective lens. To maximize the scattered light-gathering power of the objective lens, oil immersion is used and the numerical aperture (NA) of the objective lens must be less than 1.0. Objective lenses with a higher NA can be used but ...
Optical relief. Optical relief (usually noted as simply relief) is a visually observable property in optical mineralogy used to identify minerals based on their refractive index. Relief is determined by observing the degree to which grains stand out from a mounting medium of known refractive index, [1] usually either oil or Canada Balsam.
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