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Rolling circle replication (RCR) is a process of unidirectional nucleic acid replication that can rapidly synthesize multiple copies of circular molecules of DNA or RNA, such as plasmids, the genomes of bacteriophages, and the circular RNA genome of viroids. Some eukaryotic viruses also replicate their DNA or RNA via the rolling circle mechanism.
The virus replicates through an dsDNA intermediate initiated by the Rep protein. Two major genes are transcribed from open reading frame (ORF) 1 and 2. ORF1 encodes Rep and Rep' for initiation of rolling-circle replication; ORF2 encodes Cap, the only structural and most immunogenic protein forming the viral capsid. [15]
In the single stranded DNA viruses—a group that includes the circoviruses, the geminiviruses, the parvoviruses and others—and also the many phages and plasmids that use the rolling circle replication (RCR) mechanism, the RCR endonuclease creates a nick in the genome strand (single stranded viruses) or one of the DNA strands (plasmids).
2. Buckeye Lake State Park. Discover Ohio’s oldest state park, a cherished day-use gem located in Millersport. Once a feeder lake for Ohio’s canal system in the 1800s, Buckeye Lake has been a ...
The Cuyahoga Valley National Park ranks among the Black Hills of South Dakota, the Blue Ridge Parkway in Virginia and North Carolina, as well as the Adirondack and Catskill mountains in New York.
Orthopoxvirus particles. A DNA virus is a virus that has a genome made of deoxyribonucleic acid (DNA) that is replicated by a DNA polymerase.They can be divided between those that have two strands of DNA in their genome, called double-stranded DNA (dsDNA) viruses, and those that have one strand of DNA in their genome, called single-stranded DNA (ssDNA) viruses. dsDNA viruses primarily belong ...
7. Pillsbury Cinnamon Rolls, Cream Cheese Icing. 140 calories, 4.5 grams fat, 10 grams sugar. Pillsbury's rolls are studded with cinnamon-sugar nuggets that melt and spread over the top like a ...
A circular chromosome, showing DNA replication proceeding bidirectionally, with two replication forks generated at the "origin". Each half of the chromosome replicated by one replication fork is called a "replichore".