Search results
Results from the WOW.Com Content Network
Crystal structure of the Class I ligase ribozyme at 2.98 Å resolution (PDB ID: 3HHN). The phosphodiester bond formed by this enzyme is shown as spheres. The L1 Ligase Ribozyme 2.6 Å crystal structure. The RNA Ligase ribozyme was the first of several types of synthetic ribozymes produced by in vitro evolution and selection techniques.
S phase (Synthesis phase) is the phase of the cell cycle in which DNA is replicated, occurring between G 1 phase and G 2 phase. [1] Since accurate duplication of the genome is critical to successful cell division, the processes that occur during S-phase are tightly regulated and widely conserved.
In biochemistry, a ligase is an enzyme that can catalyze the joining of two molecules by forming a new chemical bond. This is typically via hydrolysis of a small pendant chemical group on one of the molecules, typically resulting in the formation of new C-O, C-S, or C-N bonds.
DNA ligase is a type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.It plays a role in repairing single-strand breaks in duplex DNA in living organisms, but some forms (such as DNA ligase IV) may specifically repair double-strand breaks (i.e. a break in both complementary strands of DNA).
The discovery of DNA ligase dates back to 1967 and was an important event in the field of molecular biology. [1] Ligation in the laboratory is normally performed using T4 DNA ligase. It is broadly used in vitro due to its capability of joining sticky-ended fragments as well as blunt-ended fragments. [2]
Eukaryotic DNA ligase 1 catalyzes a reaction that is chemically universal to all ligases. DNA ligase 1 utilizes adenosine triphosphate (ATP) to catalyze the energetically favorable ligation events in both DNA replication and repair. During the synthesis phase (S-phase) of the eukaryotic cell cycle, DNA replication occurs.
27401 Ensembl ENSG00000145604 ENSMUSG00000054115 UniProt Q13309 Q9Z0Z3 RefSeq (mRNA) NM_001243120 NM_005983 NM_032637 NM_001285980 NM_013787 NM_145468 RefSeq (protein) NP_001230049 NP_005974 NP_116026 NP_001272909 NP_038815 Location (UCSC) Chr 5: 36.15 – 36.2 Mb Chr 15: 9.11 – 9.16 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse S-phase kinase-associated protein 2 is an enzyme ...
S-phase: During cell replication when DNA is replicated, and is initiated by the S-phase-promoting factor(SPF) cyclin complexes. [2] The DNA replication takes place, due to the increase in SPF during the switching from G1 to S phase in the cell cycle. [2] SPF is also used to inhibit double replication of chromosomes in the cell cycle, which is ...