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The pour plate technique is the typical technique used to prepare plate count agars. Here, the inoculum is added to the molten agar before pouring the plate. The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample.
Colony-forming units are used to quantify results in many microbiological plating and counting methods, including: The pour plate method wherein the sample is suspended in a Petri dish using molten agar cooled to approximately 40–45 °C (just above the point of solidification to minimize heat-induced cell death).
A somatic cell count (SCC) is a cell count of somatic cells in a fluid specimen, usually milk. In dairying, the SCC is an indicator of the quality of milk—specifically, its low likeliness to contain harmful bacteria, and thus its high food safety. White blood cells (leukocytes) constitute the majority of somatic cells in question.
Spiral plating is used extensively for microbiological testing of food, milk and milk products and cosmetics. It is an approved method by the FDA. [2] The advantage of spiral plating is less plates used versus plating manually because different concentrations are present on each plate.
A third technique is using sterile glass beads to plate out cells. In this technique, cells are grown in a liquid culture, in which a small volume is pipetted on the agar plate and then spread out with the beads. Replica plating is another technique used to plate out cells on agar plates. These four techniques are the most common, but others ...
Food processing is the transformation of agricultural products into food, or of one form of food into other forms. Food processing takes many forms, from grinding grain into raw flour , home cooking , and complex industrial methods used in the making of convenience foods .
Aseptic processing was derived from Olin Ball's heat-cool-fill (HCF) machine that was developed in 1927. [5] While HCF was successful in improving the sensory quality of the processed chocolate milk as compared to canned product, the use of the equipment was hindered by its cost, maintenance, and inflexibility to process various container sizes, rendering the machine a failure.
The Neogen Petrifilm plate is an all-in-one plating system made by the Food Safety Division of the Neogen Corporation.They are heavily used in many microbiology-related industries and fields to culture various micro-organisms and are meant to be a more efficient method for detection and enumeration compared to conventional plating techniques.