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  2. Denaturation (biochemistry) - Wikipedia

    en.wikipedia.org/wiki/Denaturation_(biochemistry)

    The effects of temperature on enzyme activity. Top: increasing temperature increases the rate of reaction (Q10 coefficient). Middle: the fraction of folded and functional enzyme decreases above its denaturation temperature. Bottom: consequently, an enzyme's optimal rate of reaction is at an intermediate temperature.

  3. Q10 (temperature coefficient) - Wikipedia

    en.wikipedia.org/wiki/Q10_(temperature_coefficient)

    The effects of temperature on enzyme activity. Top - increasing temperature increases the rate of reaction (Q 10 coefficient). Middle - the fraction of folded and functional enzyme decreases above its denaturation temperature. Bottom - consequently, an enzyme's optimal rate of reaction is at an intermediate temperature.

  4. Enzyme assay - Wikipedia

    en.wikipedia.org/wiki/Enzyme_assay

    All have an optimum pH. The pH can stop enzyme activity by denaturating (altering) the three-dimensional shape of the enzyme by breaking ionic, and hydrogen bonds. Most enzymes function between a pH of 6 and 8; however pepsin in the stomach works best at a pH of 2 and trypsin at a pH of 8.

  5. Enzyme - Wikipedia

    en.wikipedia.org/wiki/Enzyme

    Enzyme activity. An enzyme's name is ... Enzyme activity initially increases with temperature ... Enzyme levels can also be regulated by changing the rate of enzyme ...

  6. Catalase - Wikipedia

    en.wikipedia.org/wiki/Catalase

    847 12359 Ensembl ENSG00000121691 ENSMUSG00000027187 UniProt P04040 P24270 RefSeq (mRNA) NM_001752 NM_009804 RefSeq (protein) NP_001743 NP_033934 Location (UCSC) Chr 11: 34.44 – 34.47 Mb Chr 2: 103.28 – 103.32 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Catalase is a common enzyme found in nearly all living organisms exposed to oxygen (such as bacteria, plants, and animals ...

  7. Thermolysin - Wikipedia

    en.wikipedia.org/wiki/Thermolysin

    At this temperature incubation for 30 minutes reduces the enzymes activity by half. Thermolysin has a T 50 value of 86.9 °C, making it the most thermo stable member of the TLP family. [ 9 ] Studies on the contribution of calcium to thermolysin stability have shown that upon thermal inactivation a single calcium ion is released from the ...

  8. Enzyme unit - Wikipedia

    en.wikipedia.org/wiki/Enzyme_unit

    The enzyme unit, or international unit for enzyme (symbol U, sometimes also IU) is a unit of enzyme's catalytic activity. [ 1 ] 1 U (μmol/min) is defined as the amount of the enzyme that catalyzes the conversion of one micro mole of substrate per minute under the specified conditions of the assay method .

  9. Taq polymerase - Wikipedia

    en.wikipedia.org/wiki/Taq_polymerase

    T. aquaticus is a bacterium that lives in hot springs and hydrothermal vents, and Taq polymerase was identified [1] as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR. [2] Therefore, it replaced the DNA polymerase from E. coli originally used in PCR. [3]