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Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase. Pyrosequencing relies on light detection based on a chain reaction when pyrophosphate is released. Hence ...
The Casio FX-7000G is a calculator which is widely known as being the world's first graphing calculator available to the public. It was introduced to the public and later manufactured between 1985 and c. 1988. [2] Notable features are its ability to graph functions, [3] and that it is programmable.
Example Bjerrum plot: Change in carbonate system of seawater from ocean acidification.. A Bjerrum plot (named after Niels Bjerrum), sometimes also known as a Sillén diagram (after Lars Gunnar Sillén), or a Hägg diagram (after Gunnar Hägg) [1] is a graph of the concentrations of the different species of a polyprotic acid in a solution, as a function of pH, [2] when the solution is at ...
Standard flowgram format (SFF) is a binary file format used to encode results of pyrosequencing from the 454 Life Sciences platform for high-throughput sequencing.SFF files can be viewed, edited and converted with DNA Baser SFF Workbench (graphic tool), or converted to FASTQ format with sff2fastq or seq_crumbs.
Phred quality scores shown on a DNA sequence trace. A Phred quality score is a measure of the quality of the identification of the nucleobases generated by automated DNA sequencing.
Stage 2 builds a splice graph representation of a gene, by connecting the exons (vertices) via introns (edges) extracted from spliced read alignments. Stage 3 selects a subset of the candidate transcripts encoded in the graph that can explain all the reads, using either a parsimonius (SET_COVER) or a dynamic programming optimization approach.
After reading the introduction I feel that the 454 should only be mentioned later under recent advantages. This way it appears that 454 is the only way pyrosequencing is applied. In addition, it is a bit of a repetition.CharonZ 10:06, 10 July 2007 (UTC)
GraphCalc is an open-source computer program that runs in Microsoft Windows and Linux that provides the functionality of a graphing calculator. GraphCalc includes many of the standard features of graphing calculators, but also includes some higher-end features: High resolution