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Single-cell sequencing examines the nucleic acid sequence information from individual cells with optimized next-generation sequencing technologies, providing a higher resolution of cellular differences and a better understanding of the function of an individual cell in the context of its microenvironment. [1]
Logarithmic coverage plot for the single-cell sequencing data for E. coli genome. [14] SPAdes was designed to overcome the problems associated with the assembly of single cell data as follows: [2] 1. Non-uniform coverage. SPAdes utilizes multisized de Bruijn graph which allows employing different values of k.
This single cell shows the process of the central dogma of molecular biology, which are all steps researchers are interested to quantify (DNA, RNA, and Protein).. In cell biology, single-cell analysis and subcellular analysis [1] refer to the study of genomics, transcriptomics, proteomics, metabolomics, and cell–cell interactions at the level of an individual cell, as opposed to more ...
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A list of more than 100 different single cell sequencing (omics) methods have been published. [1] The large majority of methods are paired with short-read sequencing technologies, although some of them are compatible with long read sequencing.
scLVM [117] scLVM is a modelling framework for single-cell RNA-seq data that can be used to dissect the observed heterogeneity into different sources, thereby allowing for the correction of confounding sources of variation. scM&T-Seq Parallel single-cell sequencing.
Sequencing by hybridization; Short Oligonucleotide Analysis Package; Shotgun sequencing; Single cell epigenomics; Single molecule fluorescent sequencing; Single-cell DNA template strand sequencing; Single-cell sequencing; Single-cell transcriptomics; Single-molecule magnetic sequencing; SNV calling from NGS data; SPAdes (software) Standard ...
UMIs are particularly well-suited to single-cell RNA-Seq transcriptomics, where the amount of input RNA is restricted and extended amplification of the sample is required. [ 73 ] [ 74 ] [ 75 ] Once the transcript molecules have been prepared they can be sequenced in just one direction (single-end) or both directions (paired-end).