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In addition, control of the fluid dynamics within each hollow fiber bioreactor led to further optimization of the cell culture environment. By alternating the pressure gradient across the hollow fiber membrane, media could flow back and forth between the EC side (cell compartment) and the IC side (hollow fiber lumen). This process, combined ...
CompuCell3D can model several different phenomena, including avian limb development, in vitro capillary development, adhesion-driven cell sorting, Dictyostelium discoideum, and fluid flows. The binaries and source code, as well as documentation and examples, are available at the CompuCell3D Website
Particularly exothermic fermentations may require the use of external heat exchangers. Nutrients may be continuously added to the fermenter, as in a fed-batch system, or may be charged into the reactor at the beginning of fermentation. The pH of the medium is measured and adjusted with small amounts of acid or base, depending upon the fermentation.
One of the most important features of chemostats is that microorganisms can be grown in a physiological steady state under constant environmental conditions. In this steady state, growth occurs at a constant specific growth rate and all culture parameters remain constant (culture volume, dissolved oxygen concentration, nutrient and product concentrations, pH, cell density, etc.).
Fed-batch reactor symbol. Fed-batch culture is, in the broadest sense, defined as an operational technique in biotechnological processes where one or more nutrients (substrates) are fed (supplied) to the bioreactor during cultivation and in which the product(s) remain in the bioreactor until the end of the run. [1]
Solid state fermentation uses culture substrates with low water levels (reduced water activity), which is particularly appropriate for mould. The methods used to grow filamentous fungi using solid state fermentation allow the best reproduction of their natural environment. The medium is saturated with water but little of it is free-flowing.
Cell therapy bioprocessing is a discipline that bridges the fields of cell therapy and bioprocessing (i.e., biopharmaceutical manufacturing), and is a sub-field of bioprocess engineering. The goals of cell therapy bioprocessing are to establish reproducible and robust manufacturing processes for the production of therapeutic cells.
A turbidostat is a continuous microbiological culture device, similar to a chemostat or an auxostat, which has feedback between the turbidity of the culture vessel and the dilution rate. [ 1 ] [ 2 ] The theoretical relationship between growth in a chemostat and growth in a turbidostat is somewhat complex, in part because they are similar.
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