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N-Ethylbuphedrone (also known as NEB) is a stimulant of the cathinone class that has been sold as a designer drug. [1] [2] [3] ...
Specific restriction endonucleases are used to digest DNA. The RFLP molecular marker is specific to a single fragment. Along with alleic RFLP markers, a molecular-weight size marker, in this case a DNA marker, [30] is also included on an electorphoresed agarose gel. The DNA marker allows for the size of the restriction fragments to be estimated.
Restriction digest is most commonly used as part of the process of the molecular cloning of DNA fragment into a vector (such as a cloning vector or an expression vector).The vector typically contains a multiple cloning site where many restriction site may be found, and a foreign piece of DNA may be inserted into the vector by first cutting the restriction sites in the vector as well the DNA ...
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New England Biolabs (NEB) is an American life sciences company which produces and supplies recombinant and native enzyme reagents for life science research. [2] It also provides products and services supporting genome editing , synthetic biology and next-generation sequencing . [ 3 ]
TEV protease (EC 3.4.22.44, Tobacco Etch Virus nuclear-inclusion-a endopeptidase) is a highly sequence-specific cysteine protease from Tobacco Etch Virus (TEV). [1] It is a member of the PA clan of chymotrypsin-like proteases. [2]
In 2012 a modified RAD tagging method called double digest RADseq (ddRADseq) was suggested. [10] [11] By adding a second restriction enzyme, replacing the random shearing, and a tight DNA size selection step it is possible to perform low-cost population genotyping.
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