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  2. URA3 - Wikipedia

    en.wikipedia.org/wiki/URA3

    URA3 is often used in yeast research as a "marker gene", that is, a gene to label chromosomes or plasmids. URA3 encodes Orotidine 5'-phosphate decarboxylase (ODCase) , which is an enzyme that catalyzes one reaction in the synthesis of pyrimidine ribonucleotides (a component of RNA ).

  3. Orotidine 5'-phosphate decarboxylase - Wikipedia

    en.wikipedia.org/wiki/Orotidine_5'-phosphate...

    In yeast and bacteria, OMP decarboxylase is a single-function enzyme.However, in mammals, OMP decarboxylase is part of a single protein with two catalytic activities.This bifunctional enzyme is named UMP synthase and it also catalyzes the preceding reaction in pyrimidine nucleotide biosynthesis, the transfer of ribose 5-phosphate from 5-phosphoribosyl-1-pyrophosphate to orotate to form OMP.

  4. Yeast deletion project - Wikipedia

    en.wikipedia.org/wiki/Yeast_deletion_project

    The yeast deletion project, formally the Saccharomyces Genome Deletion Project, is a project to create data for a near-complete collection of gene-deletion mutants of the yeast Saccharomyces cerevisiae. Each strain carries a precise deletion of one of the genes in the genome. This allows researchers to determine what each gene does by comparing ...

  5. 5-Fluoroorotic acid - Wikipedia

    en.wikipedia.org/wiki/5-Fluoroorotic_Acid

    5-Fluoroorotic acid (5FOA) is a fluorinated derivative of the pyrimidine precursor orotic acid.It is used in yeast genetics to select for the absence of the URA3 gene, which encodes the enzyme for the decarboxylation of 5-fluoroorotic acid to 5-fluorouracil, a toxic metabolite. [1]

  6. Synthetic genetic array - Wikipedia

    en.wikipedia.org/wiki/Synthetic_genetic_array

    Synthetic genetic array analysis is generally conducted using colony arrays on petriplates at standard densities (96, 384, 768, 1536). To perform a SGA analysis in S.cerevisiae, the query gene deletion is crossed systematically with a deletion mutant array (DMA) containing every viable knockout ORF of the yeast genome (currently 4786 strains). [9]

  7. Y187 - Wikipedia

    en.wikipedia.org/wiki/Y187

    Y187 is a strain of yeast (Saccharomyces cerevisiae) used in biological research for two-hybrid screening.The strain has been sold commercially by Clontech [1] since at least 2000 and is used as a partner with strain AH109 in mating assays.

  8. Selectable marker - Wikipedia

    en.wikipedia.org/wiki/Selectable_marker

    A selectable marker is a gene introduced into cells, especially bacteria or cells in culture, which confers one or more traits suitable for artificial selection.They are a type of reporter gene used in laboratory microbiology, molecular biology, and genetic engineering to indicate the success of a transfection or transformation or other procedure meant to introduce foreign DNA into a cell.

  9. Gene knockout - Wikipedia

    en.wikipedia.org/wiki/Gene_knockout

    A conditional gene knockout allows gene deletion in a tissue in a tissue specific manner. This is required in place of a gene knockout if the null mutation would lead to embryonic death, [13] or a specific tissue or cell type is of specific interest. This is done by introducing short sequences called loxP sites around the gene.

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