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The theory of high performance liquid chromatography-HPLC is, at its core, the same as general chromatography theory. [41] This theory has been used as the basis for system-suitability tests, as can be seen in the USP Pharmacopaeia, [ 42 ] which are a set of quantitative criteria, which test the suitability of the HPLC system to the required ...
Liquid chromatography (LC) is a separation technique in which the mobile phase is a liquid. It can be carried out either in a column or a plane. Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as high-performance liquid chromatography.
Reversed-phase liquid chromatography (RP-LC) is a mode of liquid chromatography in which non-polar stationary phase and polar mobile phases are used for the separation of organic compounds. [ 1 ] [ 2 ] [ 3 ] The vast majority of separations and analyses using high-performance liquid chromatography (HPLC) in recent years are done using the ...
Liquid chromatography is a method of physical separation in which the components of a liquid mixture are distributed between two immiscible phases, i.e., stationary and mobile. The practice of LC can be divided into five categories, i.e., adsorption chromatography , partition chromatography , ion-exchange chromatography , size-exclusion ...
A monolithic HPLC column, or monolithic column, is a column used in high-performance liquid chromatography (HPLC). The internal structure of the monolithic column is created in such a way that many channels form inside the column. The material inside the column which separates the channels can be porous and functionalized.
The charged aerosol detector (CAD) is a detector used in conjunction with high-performance liquid chromatography (HPLC) and ultra high-performance liquid chromatography (UHPLC) to measure the amount of chemicals in a sample by creating charged aerosol particles which are detected using an electrometer.
In recent years, high performance liquid chromatography online coupled to mass spectrometry became very popular. By choosing porous graphitic carbon as a stationary phase for liquid chromatography, even non derivatized glycans can be analyzed. Electrospray ionisation is frequently used for this application. [13] [14] [15]
A chromatography detector is a device that detects and quantifies separated compounds as they elute from the chromatographic column.These detectors are integral to various chromatographic techniques, such as gas chromatography, [1] liquid chromatography, and high-performance liquid chromatography, [2] and supercritical fluid chromatography [3] among others.