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In order to achieve temperatures lower than liquid helium at atmospheric pressure, additional cooler stages may be added to the cryostat. Temperatures down to 1 K can be reached by attaching the cold plate to a 1-K pot, which is a container of the He-4 isotope that may be pumped to low vapor pressure via a vacuum pump. Temperatures just below 0 ...
A scientist placing samples into a negative 80 degree freezer. In contrast to short term sample storage at +4 to −20 °C (39 to −4 °F) by using standard refrigerators or freezers, many molecular biology or life science laboratories need long-term cryopreservation (including "cold chain" and/or "colder chain" infrastructures) for biological samples like DNA, RNA, proteins, cell extracts ...
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The Joule-Thomson (JT) cooler was invented by Carl von Linde and William Hampson so it is also called the Linde-Hampson cooler. It is a simple type of cooler which is widely applied as cryocooler or as the (final stage) of coolants. It can easily be miniaturized, but it is also used on a very large scale in the liquefaction of natural gas.
Domestic freezers can be included as a separate compartment in a refrigerator, or can be a separate appliance. Domestic freezers may be either upright, resembling a refrigerator, or chest freezers, wider than tall with the lid or door on top, sacrificing convenience for efficiency and partial immunity to power outages. [29]
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