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Alginate was discovered by British chemical scientist E. C. C. Stanford in 1881, and he patented an extraction process for it in the same year. [4] The alginate was extracted, in the original patent, by first soaking the algae in water or diluted acid, then extracting the alginate by soaking it in sodium carbonate , and finally precipitating ...
Perstraction is the separation technique developed from liquid-liquid extraction. Due to the presence of the membrane a wider selection of extractants can be used, this can include the use of miscible solutions, for example the recovery of ammonia from waste water using sulphuric acid.
Download as PDF; Printable version; ... Alginate; Alginate is an ... all of these rely on extraction methods through hardwood or softwood trees milled into smaller ...
Adsorption on glass, alginate beads or matrix: Enzyme is attached to the outside of an inert material. In general, this method is the slowest among those listed here. As adsorption is not a chemical reaction, the active site of the immobilized enzyme may be blocked by the matrix or bead, greatly reducing the activity of the enzyme. [9]
Unlike enzyme immobilization, where the enzyme is attached to a solid support (such as calcium alginate or activated PVA or activated PEI), in immobilized whole cell systems, the target cell is immobilized. Such methods may be implemented when the enzymes required are difficult or expensive to extract, an example being intracellular enzymes.
[2] [26] Other methods for liquid extraction include a soft-extraction with low temperature milling to suspend fine particles in water, heating the raw material with alkaline sodium or potassium to extract nutrients and the addition of enzymes to aid in biochemical decomposition.
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Calcium alginate is a water-insoluble, gelatinous, cream-coloured substance that can be created through the addition of aqueous calcium chloride to aqueous sodium alginate. Calcium alginate is also used for entrapment of enzymes and forming artificial seeds in plant tissue culture.