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Weigert's elastic stain is a combination of stains used in histology which is useful in identifying elastic fibers. Often orcein or a combination of resorcinol and fuchsine are used for staining. For counterstaining cell nuclei nuclear fast red or hematoxylin is also used. After applying, elastic fibers show up blue colored while cell nuclei ...
Many methods of performing Perls Prussian blue stain for iron have been published, [2] Drury and Wallington (1980) give a protocol that uses a mixture of 1 part 2% hydrochloric acid and 1 part 2% potassium ferrocyanide that is applied to the section for 20–30 minutes followed by a rinse in distilled water and application of a counterstain ...
Another common variant is the Masson trichrome & Verhoeff stain, which combines the Masson trichrome stain and Verhoeff's stain. [2] This combination is useful for the examination of blood vessels ; the Verhoeff stain highlights elastin (black) and allows one to easily differentiate small arteries (which typically have at least two elastic ...
Due to its short staining time, Diff-Quik stain is often used for initial screening of cytopathology specimens. This staining technique allows the cytotechnologist or pathologist to quickly assess the adequacy of the specimen, identify possible neoplastic or inflammatory changes, and decide whether or not additional staining is required. [4] [9 ...
Immunohistochemistry or IHC staining of tissue sections (or immunocytochemistry, which is the staining of cells), is perhaps the most commonly applied immunostaining technique. [2] While the first cases of IHC staining used fluorescent dyes (see immunofluorescence ), other non-fluorescent methods using enzymes such as peroxidase (see ...
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