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Although it could be used in other formats (e.g., Petri plates), Simmons' citrate agar is often used in slants or slopes in test tubes. One advantage of using slants over Petri dishes is access to oxygen, which is required by citrate metabolism. Simmons’ agar can be bought from suppliers as ready-made powders or slants.
TCBS agar contains high concentrations of sodium thiosulfate and sodium citrate to inhibit the growth of Enterobacteriaceae. Inhibition of gram-positive bacteria is achieved by the incorporation of ox gall , which is a naturally occurring substance containing a mixture of bile salts and sodium cholate , a pure bile salt .
The TSI slant is a test tube that contains agar, a pH-sensitive dye , 1% lactose, 1% sucrose, 0.1% glucose, [2] and sodium thiosulfate and ferrous sulfate or ferrous ammonium sulfate. All of these ingredients are mixed together, heated to sterility, and allowed to solidify in the test tube at a slanted angle.
Apocholate citrate agar (ACA) is a selective environment used to isolate Shigella and Salmonella bacteria. [1] The name derives from apocholate and citrate in agar.
Once a plate has been successfully prepared, plate count agar cells will grow into colonies which can be sufficiently isolated to determine the original cell type. The colony-forming unit (CFU) is an appropriate description of the colony's origin. In plate counts, colonies are counted, but the count is usually recorded in CFU.
Mueller Hinton agar is commonly used in the disk diffusion method, which is a simple and widely used method for testing the susceptibility of bacterial isolates to antibiotics. In this method, small disks impregnated with different antibiotics are placed on the surface of the agar, and the zone of inhibition around each disk is measured to ...
In order to prepare 1 liter of the Farrell's medium, the following quantities are added to 1 liter of serum dextrose agar: polymyxin B sulfate (5000 units =5 mg), bacitracin (25,000 units = 25 mg), natamycin (50 mg), nalidixic acid (5 mg), nystatin (100,000 units), and vancomycin (20 mg).
De Man–Rogosa–Sharpe agar, often abbreviated to MRS, is a selective culture medium designed to favour the luxuriant growth of Lactobacilli for lab study. Developed in 1960, this medium was named for its inventors, Johannes Cornelis de Man [ Wikidata ] , Morrison Rogosa [ Wikidata ] , and Margaret Elisabeth Sharpe [ Wikidata ] .
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