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Although it could be used in other formats (e.g., Petri plates), Simmons' citrate agar is often used in slants or slopes in test tubes. One advantage of using slants over Petri dishes is access to oxygen, which is required by citrate metabolism. Simmons’ agar can be bought from suppliers as ready-made powders or slants.
The TSI slant is a test tube that contains agar, a pH-sensitive dye , 1% lactose, 1% sucrose, 0.1% glucose, [2] and sodium thiosulfate and ferrous sulfate or ferrous ammonium sulfate. All of these ingredients are mixed together, heated to sterility, and allowed to solidify in the test tube at a slanted angle.
The slant culture cap is then removed and secured using the needle hand. Flaming the open end of the slant culture will prevent contamination and the formation of aerosols. [2] [3] Transfer happens once the tip of the inoculation needle comes into contact with the agar surface of the slant culture. The inoculation needle should not move in the ...
Bromothymol blue was added in order to reduce false positives. The citrate agar is green before inoculation, and turns blue, because of BTB as a positive test indicator, meaning citrate is utilized. The test is also prepared on a slant to maximize bacterial growth for an even better indication of the use of citrate.
Thiosulfate–citrate–bile salts–sucrose agar, or TCBS agar, is a type of selective agar culture plate that is used in microbiology laboratories to isolate Vibrio species. [1] TCBS agar is highly selective for the isolation of V. cholerae and V. parahaemolyticus as well as other Vibrio species. Apart from TCBS agar, other rapid testing ...
As there are many bacteria that also look like Salmonella on DCA, it is widely recommended that more selective agars are used for the identification of Salmonella, namely xylose lysine deoxycholate (XLD) agar. This growth medium is heat-sensitive and should be poured and cooled as soon as possible after addition of the deoxycholate, otherwise ...
Apocholate citrate agar (ACA) is a selective environment used to isolate Shigella and Salmonella bacteria. [1] The name derives from apocholate and citrate in agar.
De Man–Rogosa–Sharpe agar, often abbreviated to MRS, is a selective culture medium designed to favour the luxuriant growth of Lactobacilli for lab study. Developed in 1960, this medium was named for its inventors, Johannes Cornelis de Man [ Wikidata ] , Morrison Rogosa [ Wikidata ] , and Margaret Elisabeth Sharpe [ Wikidata ] .