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pICalculax — Isoelectric point (pI) predictor for chemically modified peptides and proteins; SWISS-2DPAGE Archived 2016-12-10 at the Wayback Machine — a database of isoelectric points coming from two-dimensional polyacrylamide gel electrophoresis (~ 2,000 proteins) PIP-DB — a Protein Isoelectric Point database (~ 5,000 proteins)
Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different molecules by differences in their isoelectric point (pI). [ 1 ] [ 2 ] It is a type of zone electrophoresis usually performed on proteins in a gel that takes advantage of the fact that overall charge on the molecule of interest is a function of the ...
Leucine (symbol Leu or L) [3] is an essential amino acid that is used in the biosynthesis of proteins.Leucine is an α-amino acid, meaning it contains an α-amino group (which is in the protonated −NH 3 + form under biological conditions), an α-carboxylic acid group (which is in the deprotonated −COO − form under biological conditions), and a side chain isobutyl group, making it a non ...
LRRC27 Isoform a in humans is 530 amino acids long with a predicted molecular weight of ~60 kDa and predicted isoelectric point around 9. [6] It is relatively poor in tyrosine compared to other proteins. [7] LRRC27 is also largely localized in the nucleus of a cell. [8]
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A typical crossing time for a sensor platform is 10–30 s (an 8 m web, 60 cm/s). The sensor platform scans across the paper web and continuously measures paper characteristics from edge to edge. It can also be directed and stopped to a specific, fixed point on the web to measure the machine-direction (MD) variation at a single point.