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A calibration curve plot showing limit of detection (LOD), limit of quantification (LOQ), dynamic range, and limit of linearity (LOL).. In analytical chemistry, a calibration curve, also known as a standard curve, is a general method for determining the concentration of a substance in an unknown sample by comparing the unknown to a set of standard samples of known concentration. [1]
In summary, in order to find a standard curve, one must use varying concentrations of BSA (Bovine Serum Albumin) [2] in order to create a standard curve with concentration plotted on the x-axis and absorbance plotted on the y-axis. Only a narrow concentration of BSA is used (2-10 ug/mL) in order to create an accurate standard curve. [23]
The Standard addition method, often used in analytical chemistry, quantifies the analyte present in an unknown. This method is useful for analyzing complex samples where a matrix effect interferes with the analyte signal. In comparison to the calibration curve method, the standard addition method has the advantage of the matrices of the unknown ...
Definitions. The Lineweaver–Burk plot derives from a transformation of the Michaelis–Menten equation, in which the rate is a function of the substrate concentration and two parameters , the limiting rate, and , the Michaelis constant. Taking reciprocals of both sides of this equation it becomes as follows:
Standard solutions are commonly used to determine the concentration of an analyte species via calibration curve. A calibration curve is obtained by measuring a series of standard solutions with known concentrations, which can be used to determine the concentration of an unknown sample using linear regression analysis. [4]
These three equations show that the curves for CO 2 and HCO − 3 intersect at [H +] eq = K 1, and the curves for HCO − 3 and CO 2− 3 intersect at [H +] eq = K 2. Therefore, the values of K 1 and K 2 that were used to create a given Bjerrum plot can easily be found from that plot, by reading off the concentrations at these points of ...
A titration curve is a curve in graph the x-coordinate of which represents the volume of titrant added since the beginning of the titration, and the y-coordinate of which represents the concentration of the analyte at the corresponding stage of the titration (in an acid–base titration, the y-coordinate usually represents the pH of the solution).
The calibration curve that does not use the internal standard method ignores the uncertainty between measurements. The coefficient of determination (R 2 ) for this plot is 0.9985. In the calibration curve that uses the internal standard, the y-axis is the ratio of the nickel signal to the yttrium signal.