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  2. Recombinant DNA - Wikipedia

    en.wikipedia.org/wiki/Recombinant_DNA

    Recombinant DNA molecules are sometimes called chimeric DNA because they can be made of material from two different species like the mythical chimera. rDNA technology uses palindromic sequences and leads to the production of sticky and blunt ends. The DNA sequences used in the construction of recombinant DNA molecules can originate from any ...

  3. Molecular cloning - Wikipedia

    en.wikipedia.org/wiki/Molecular_cloning

    Molecular cloning takes advantage of the fact that the chemical structure of DNA is fundamentally the same in all living organisms. Therefore, if any segment of DNA from any organism is inserted into a DNA segment containing the molecular sequences required for DNA replication, and the resulting recombinant DNA is introduced into the organism from which the replication sequences were obtained ...

  4. In vitro recombination - Wikipedia

    en.wikipedia.org/wiki/In_vitro_recombination

    Recombinant DNA (rDNA), or molecular cloning, is the process by which a single gene, or segment of DNA, is isolated and amplified. Recombinant DNA is also known as in vitro recombination. A cloning vector is a DNA molecule that carries foreign DNA into a host cell, where it

  5. Fragmentation (cell biology) - Wikipedia

    en.wikipedia.org/wiki/Fragmentation_(cell_biology)

    The key to cloning a DNA fragment is to link it to a vector DNA molecule that can replicate within a host cell. After a single recombinant DNA molecule (composed of a vector plus an inserted DNA fragment) is introduced into a host cell, the inserted DNA can be replicated along with the vector, generating a large number of identical DNA ...

  6. Genetic recombination - Wikipedia

    en.wikipedia.org/wiki/Genetic_recombination

    In genetic engineering, recombination can also refer to artificial and deliberate recombination of disparate pieces of DNA, often from different organisms, creating what is called recombinant DNA. A prime example of such a use of genetic recombination is gene targeting , which can be used to add, delete or otherwise change an organism's genes.

  7. Homologous recombination - Wikipedia

    en.wikipedia.org/wiki/Homologous_recombination

    The SSA pathway is unique in that it does not require a separate similar or identical molecule of DNA, like the DSBR or SDSA pathways of homologous recombination. Instead, the SSA pathway only requires a single DNA duplex, and uses the repeat sequences as the identical sequences that homologous recombination needs for repair.

  8. Our DNA is 99.9 percent the same as the person sitting next ...

    www.aol.com/article/2016/05/06/our-dna-is-99-9...

    Our bodies have 3 billion genetic building blocks, or base pairs, that make us who we are. And of those 3 billion base pairs, only a tiny amount are unique to us, making us about 99.9% genetically ...

  9. Microbial genetics - Wikipedia

    en.wikipedia.org/wiki/Microbial_Genetics

    The usage of recombinant DNA technology is a process of this work. [1] The process involves creating recombinant DNA molecules through manipulating a DNA sequence. [1] That DNA created is then in contact with a host organism. Cloning is also an example of genetic engineering. [1]