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Structural overview of T2 phage. T4 is a relatively large virus, at approximately 90 nm wide and 200 nm long (most viruses range from 25 to 200 nm in length). The DNA genome is held in an icosahedral head, also known as a capsid. [9] The T4's tail is hollow so that it can pass its nucleic acid into the cell
This family includes lambda phage lysozyme and Escherichia coli T4 phage endolysin. [8] Lysozyme helps to release mature phage particles from the cell wall by breaking down the peptidoglycan . The enzyme hydrolyses the 1,4-beta linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of prokaryotic cell ...
15 T4 phage lysozyme. 16 Immunoglobulins. 17 Superoxide dismutase. 18 Transfer RNA. ... it is the favorite test case for new crystallographic experiments and instruments.
Structural model at atomic resolution of bacteriophage T4 [1] The structure of a typical myovirus bacteriophage Anatomy and infection cycle of bacteriophage T4.. A bacteriophage (/ b æ k ˈ t ɪər i oʊ f eɪ dʒ /), also known informally as a phage (/ ˈ f eɪ dʒ /), is a virus that infects and replicates within bacteria and archaea.
There are three classes of genes in the phage genome that regulate whether the lytic or lysogenic cycles will emerge. The first class is the immediate early genes, the second is the delayed early genes and the third is the late genes. The following refers to the well-studied temperate phage lambda of E. coli. [citation needed]
Although the endolysin of phage T4 encoded by the e gene (Lysozyme E) was identified in 1961, the holin (product of gene t and called T-holin) was not characterized until 2001. [1] A representative list of proteins belonging to the T4 holin family can be found in the Transporter Classification Database .
T4 lysozyme ribbon schematic (from PDB 1LZM) Brian W. Matthews is a biochemist and biophysicist educated at the University of Adelaide, contributor to x-ray crystallographic methodology [4] at the University of Cambridge, and since 1970 at the University of Oregon as Professor of Physics and HHMI investigator in the Institute of Molecular Biology.
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